Polyethylene glycol-potassium phosphate aqueous two-phase systems for countercurrent chromatography of proteins

被引：11
|
作者：
Shibusawa, Y
Yamaguchi, M
Ito, Y
机构：
[1] Tokyo Univ Pharm & Life Sci, Sch Pharm, Dept Analyt Chem, Tokyo 19203, Japan
[2] NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 1998年 / 21卷 / 1-2期
关键词：
D O I：
10.1080/10826079808001941
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Aqueous-aqueous polymer phase systems composed of polyethylene glycol 1000-potassium phosphate were used for the countercurrent chromatographic separation of proteins using the type XL cross-axis coil planet centrifuge. It was found that the peak resolution of proteins is highly dependant on the pH of the solvent system. The best separation of cytochrome c, myoglobin and human serum albumin was achieved with 16.0% PEG 1000-12.5% potassium phosphate buffer at pH 9.2. The resolution and separation time were improved by pH-peak-focusing countercurrent chromatography.
引用
收藏
页码：121 / 133
页数：13
相关论文
共 50 条
  • [21] Partitioning behavior of amino acids in aqueous two-phase systems containing polyethylene glycol and phosphate buffer
    Shang, QK
    Li, W
    Jia, Q
    Li, DQ
    FLUID PHASE EQUILIBRIA, 2004, 219 (02) : 195 - 203
  • [22] Selection and Use of Poly Ethylene Glycol and Phosphate Based Aqueous Two-Phase Systems for the Separation of Proteins by Centrifugal Partition Chromatography
    Schwienheer, Christoph
    Merz, Juliane
    Schembecker, Gerhard
    JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2015, 38 (09) : 929 - 941
  • [23] Purification of extracellular α-amylase from Bacillus subtilis by partitioning in a polyethylene glycol/potassium phosphate aqueous two-phase system
    Nguyen Hoang Loc
    Nguyen Thi Thuy Mien
    Do Thi Bich Thuy
    ANNALS OF MICROBIOLOGY, 2010, 60 (04) : 623 - 628
  • [24] Purification of extracellular α-amylase from Bacillus subtilis by partitioning in a polyethylene glycol/potassium phosphate aqueous two-phase system
    Nguyen Hoang Loc
    Nguyen Thi Thuy Mien
    Do Thi Bich Thuy
    Annals of Microbiology, 2010, 60 : 623 - 628
  • [25] Partition of whey milk proteins in aqueous two-phase systems of polyethylene glycol-phosphate as a starting point to isolate proteins expressed in transgenic milk
    Capezio, L
    Romanini, D
    Picó, GA
    Nerli, B
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2005, 819 (01): : 25 - 31
  • [26] Differences in Cerrena unicolor and Pleurotus sapidus laccase partitioning in polyethylene glycol - phosphate aqueous two-phase systems
    Blatkiewicz, Michal
    Antecka, Anna
    Gorak, Andrzej
    Ledakowicz, Stanislaw
    NEW BIOTECHNOLOGY, 2016, 33 : S28 - S28
  • [27] Features of the acid protease partition in aqueous two-phase systems of polyethylene glycol-phosphate:: Chymosin and pepsin
    Spelzini, D
    Farruggia, B
    Picó, G
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2005, 821 (01): : 60 - 66
  • [28] THERMODYNAMICS OF POTASSIUM HYDROGEN PHOSPHATE POTASSIUM DIHYDROGEN PHOSPHATE POLYETHYLENE-GLYCOL AQUEOUS 2-PHASE SYSTEMS
    PENG, QH
    LI, ZC
    LI, YG
    FLUID PHASE EQUILIBRIA, 1994, 95 : 341 - 357
  • [29] Partitioning of biomolecules in aqueous two-phase systems of polyethylene glycol and nonionic surfactant
    Liu, Yang
    Wu, Zhongyang
    Zhang, Yanglong
    Yuan, Haihua
    BIOCHEMICAL ENGINEERING JOURNAL, 2012, 69 : 93 - 99
  • [30] Search for new aqueous-aqueous two-phase systems for the countercurrent chromatographic separation of proteins
    Takeuchi, N
    Takeshige, S
    Nagatsuka, W
    Shindo, H
    Shibusawa, Y
    BUNSEKI KAGAKU, 2004, 53 (09) : 899 - 904
12345