Twin prime editor: seamless repair without damage

被引：6

作者：

Awan, Muhammad Jawad Akbar ^{[1
]}

Ali, Zahir ^{[2
]}

Amin, Imran ^{[1
]}

Mansoor, Shahid ^{[1
]}

机构：

[1] Pakistan Inst Engn & Appl Sci, Constituent Coll, Natl Inst Biotechnol & Genet Engn NIBGE, Agr Biotechnol Div, Jhang Rd, Faisalabad, Pakistan

[2] 4700 King Abdullah Univ Sci & Technol, Div Biol Sci, Lab Genome Engn & Synthet Biol, Thuwal 239556900, Saudi Arabia

来源：

TRENDS IN BIOTECHNOLOGY | 2022年 / 40卷 / 04期

关键词：

D O I：

10.1016/j.tibtech.2022.01.013

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

CRISPR-Cas9 creates remarkable possibilities to modify targeted regions in genomic DNA. However, CRISPR-Cas-mediated DNA double-stranded breaks (DSBs), that tend to generate random insertions or deletions, limit this technology. Recently, Anzalone et al. developed a 'twin prime editing' tool to replace, integrate, or delete large genomic DNA sequences without generating DNA DSBs.

引用

页码：374 / 376

页数：3

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