MiR-29a and Messenger RNA Expression of Bone Turnover Markers in Canonical Wnt Pathway in Patients with Ankylosing Spondylitis

被引:19
作者
Huang, Jinxian [1 ]
Song, Guoxiang [2 ]
Yin, Zhihua [3 ]
Fu, Zhongchao [3 ]
Ye, Zhizhong [3 ]
机构
[1] Univ Hong Kong, Shenzhen Hosp, Rheumatol Dept, 1st Haiyuan Rd, Shenzhen 518000, Peoples R China
[2] Third Peoples Hosp Shenzhen, Shenzhen, Peoples R China
[3] Fourth Peoples Hosp Shenzhen, Rheumatol Dept, Shenzhen, Peoples R China
基金
中国国家自然科学基金;
关键词
ankylosing spondylitis; MiR-29a; mRNA; canonical Wnt pathway; CONDITIONAL ACTIVATION; DKK-1; DICKKOPF-1; LEADS; OSTEOBLASTS; SCLEROSTIN; BIOMARKERS; DEPOSITION; LEVEL;
D O I
10.7754/Clin.Lab.2017.161214
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Background: Recent studies showed that the canonical Wnt pathway and miR-29a play important roles in the pathogenesis of bone formation. We studied the levels of miR-29a and messenger RNA (mRNA) of bone turnover markers in the canonical Wnt pathway in ankylosing spondylitis (AS). Methods: The levels of miR-29a and mRNA of bone turnover markers in canonical Wnt pathway from peripheral blood mononuclear cells were determined by real-time quantitative polymerase chain reaction in 38 patients with AS and 32 healthy controls. Correlation analysis was conducted between the levels of miR-29a and mRNA and clinical measurements using Spearman's correlation test. Results: Compared to healthy controls, the levels of miR-29a, Dickkopf (DKK)-1, P-catenin and Runx2 mRNA were significantly higher in AS patients (p < 0.05). In contrast, the levels of Gsk-3 beta mRNA was significantly lower in AS patients than that in healthy controls (p < 0.05). Gsk-3 beta mRNA was positively correlated with beta-catenin mRNA expression (p < 0.05) and no other correlation was observed between any other markers (p > 0.05). Only DKK-1 mRNA expression was negatively correlated with disease course (p < 0.05) and no other correlation was observed between markers and clinical measurements (p > 0.05). Conclusions: The osteoblastic marker miR-29a and downstream mRNA of canonical Wnt signaling was upregulated in AS, suggesting their possible role in new bone formation in AS.
引用
收藏
页码:955 / 960
页数:6
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