Isolation and characterization of a rice cysteine protease gene, OSCP1, using T-DNA gene-trap system

被引：95
作者：
Lee, S
Jung, KH
An, GH
Chung, YY [1 ]
机构：
[1] Korea Univ, Sch Life Sci & Biotechnol, Seoul 136701, South Korea
[2] Pohang Univ Sci & Technol, Div Mol & Life Sci, Pohang 790784, South Korea
来源：
PLANT MOLECULAR BIOLOGY | 2004年 / 54卷 / 05期
关键词：
anther; cell death; cysteine protease; rice; tapetum; T-DNA insertional mutagenesis;
D O I：
10.1023/B:PLAN.0000040904.15329.29
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The T-DNA gene-trap system has been efficiently used to elucidate gene functions in plants. We report here a functional analysis of a cysteine protease gene, OsCP1, isolated from a pool of T-DNA insertional rice. GUS assay with the T-DNA tagged line indicated that the OsCP1 promoter was highly active in the rice anther. Sequence analysis revealed that the deduced amino acid sequence of OsCP1 was homologous to those of papain family cyteine proteases containing the highly conserved interspersed amino acid motif, ERFNIN. This result suggested that the gene encodes a cysteine protease in rice. We also identified a suppressed mutant from T2 progeny of the T-DNA tagged line. The mutant showed a significant defect in pollen development. Taken together, the results demonstrated that OsCP1 is a cysteine protease gene that might play an important role in pollen development.
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页码：755 / 765
页数：11
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