In situ quantification of HER2-protein tyrosine kinase 6 (PTK6) protein-protein complexes in paraffin sections from breast cancer tissues

被引：25

作者：

Aubele, M. ^{[1
]}

Spears, M. ^{[2
,3
]}

Ludyga, N. ^{[1
]}

Braselmann, H. ^{[4
]}

Feuchtinger, A. ^{[1
]}

Taylor, K. J. ^{[2
,3
]}

Lindner, K. ^{[1
]}

Auer, G. ^{[5
]}

Stering, K. ^{[1
]}

Hoefler, H. ^{[1
,6
]}

Schmitt, M. ^{[7
]}

Bartlett, J. M. S. ^{[2
,3
]}

机构：

[1] Helmholtz Zentrum Munchen, German Res Ctr Environm Hlth, Inst Pathol, D-85764 Neuherberg, Germany

[2] Western Gen Hosp, Endocrine Canc Grp, Edinburgh EH4 2XR, Midlothian, Scotland

[3] Western Gen Hosp, Canc Res Ctr, Breakthrough Res Unit, Edinburgh EH4 2XR, Midlothian, Scotland

[4] Helmholtz Zentrum Munchen, German Res Ctr Environm Hlth, Dept Radiat Cytogenet, D-85764 Neuherberg, Germany

[5] Karolinska Hosp & Inst, Dept Pathol & Oncol, S-17176 Stockholm, Sweden

[6] Tech Univ Munich, Inst Pathol, D-81675 Munich, Germany

[7] Tech Univ Munich, Dept Obstet & Gynecol, Clin Res Unit, D-81675 Munich, Germany

来源：

BRITISH JOURNAL OF CANCER | 2010年 / 103卷 / 05期

关键词：

PTK6 (BRK); HER2; PLA; protein-protein complexes; formalin-fixed paraffin-embedded (FFPE) breast cancer tissue; MAMMARY EPITHELIAL-CELLS; EPIDERMAL-GROWTH-FACTOR; LONG-TERM SURVIVAL; PROXIMITY LIGATION; TUMOR KINASE; BRK; PROLIFERATION; CARCINOMAS; EXPRESSION; TARGET;

D O I：

10.1038/sj.bjc.6605836

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

BACKGROUND: Protein tyrosine kinase 6 (PTK6; breast tumour kinase) is overexpressed in up to 86% of the invasive breast cancers, and its association with the oncoprotein human epidermal growth factor receptor 2 (HER2) was shown in vitro by co-precipitation. Furthermore, expression of PTK6 in tumours is linked with the expression of HER2. METHOD AND RESULTS: In this study, we used the proximity ligation assay (PLA) technique on formalin-fixed paraffin sections from eighty invasive breast carcinoma tissue specimens to locate PTK6-HER2 protein-protein complexes. Proximity ligation assay signals from protein complexes were assessed quantitatively, and expression levels showed a statistically significant association with tumour size (P=0.015) and course of the cancer disease (P=0.012). CONCLUSION: Protein tyrosine kinase 6 forms protein complexes with HER2 in primary breast cancer tissues, which can be visualised by use of the PLA technique. Human epidermal growth factor receptor 2-PTK6 complexes are of prognostic relevance. British Journal of Cancer (2010) 103, 663-667. doi:10.1038/sj.bjc.6605836 www.bjcancer.com Published online 10 August 2010 (C) 2010 Cancer Research UK

引用

页码：663 / 667

页数：5

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