Controlled release of stromal cell-derived factor-1α from silk fibroin-coated coils accelerates intra-aneurysmal organization and occlusion of neck remnant by recruiting endothelial progenitor cells

被引:1
作者
Gao, Yuyuan [1 ]
Wang, Qiujing [1 ]
Cui, Xubo [1 ]
Liu, Yaqi [1 ]
Zheng, Tao [1 ]
Chen, Chengwei [1 ]
Sun, Chengmei [1 ]
Huang, Shuyun [1 ]
Wang, Xin [1 ]
Liu, Yanchao [1 ]
Jiang, Xiaodan [1 ]
Zeng, Chi [2 ]
Quan, Daping [2 ]
机构
[1] Southern Med Univ, Zhujiang Hosp, Dept Neurosurg,Guangdong Prov Key Lab Brain Funct, Natl Key Clin Specialty,Neurosurg Inst Guangdong, Guangzhou 510282, Guangdong, Peoples R China
[2] Sun Yat Sen Univ, Sch Chem & Chem Engn, Guangzhou 510275, Guangdong, Peoples R China
来源
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY | 2014年 / 7卷 / 12期
关键词
Stromal cell-derived factor-1 alpha; endothelial progenitor cells; silk fibroin; cerebral aneurysm; recanalization; endothelialization; RUPTURED INTRACRANIAL ANEURYSMS; GROWTH-FACTOR; ENDOVASCULAR COILING; CEREBRAL ANEURYSMS; PLATINUM COILS; RAT MODEL; IN-VITRO; PROLIFERATION; EMBOLIZATION; MOBILIZATION;
D O I
暂无
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
This study is to test the efficacy of stromal cell-derived factor-1 alpha (SDF-1 alpha)-coated coils together with endothelial progenitor cells (EPCs) transplantation in occluding aneurysms. Bone marrow-derived EPC surface markers were analyzed using flow cytometry. The migratory function of EPCs in response to SDF-1 alpha was evaluated using a modified Boyden chamber assay. Capillary-like tube formation was assessed using Matrigel gel. Coil morphologies before and after coating with SDF-1 alpha were observed under a scanning electron microscope. The level of SDF-1 alpha in supernatants was measured by ELISA. Sprague-Dawley rats were randomly allocated into five groups. Histological analysis was performed on days 14 and 28 after coil implantation. The bone marrow-EPCs could express CD133, CD34, and VEGFR-2 and form tubule-like structures in vitro. Migratory ability of EPCs in the presence of SDF-1 alpha-coated coils was similar to that in the presence of 5 ng/ml SDF-1 alpha gradient. Sustained release of SDF-1 alpha was achieved using silk fibroin as a carrier. In SDF-1 alpha-coated coils + EPCs transplantation group, a well-organized fibrous tissue bridging the orifice of aneurysms was shown on days 14 and 28. On day 28, tissue organization was greater in the SDF-1 alpha-coated coils group than in the unmodified coils group. Immunofluorescence showed a-smooth muscle actin-positive cells in organized tissue in sacs. Combined treatment with SDF-1 alpha-coated coils and EPCs transplantation is a safe and effective treatment for rat aneurysms. This may provide a new strategy for endovascular therapy following aneurysmal subarachnoid hemorrhage.
引用
收藏
页码:8366 / 8380
页数:15
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