Vaccinia virus regulates expression of p21WAF1/Cip1 in A431 cells

被引：1

作者：

Andrade, Anderson A. ^{[1
,2
]}

Brasil, Bruno S. A. F. ^{[1
,2
]}

Pereira, Anna C. T. C. ^{[3
]}

Ferreira, Paulo C. P. ^{[2
]}

Kroon, Erna G. ^{[2
]}

Bonjardim, Claudio A. ^{[1
,2
]}

机构：

[1] Univ Fed Minas Gerais, Inst Ciencias Biol, Grp Transducao Sinal, BR-31270901 Belo Horizonte, MG, Brazil

[2] Univ Fed Minas Gerais, Inst Ciencias Biol, Virus Lab, Dept Microbiol, BR-31270901 Belo Horizonte, MG, Brazil

[3] Univ Fed Piaui, Parnaiba, PI, Brazil

来源：

MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2010年 / 105卷 / 03期

关键词：

Vaccinia virus; virus-host cell interaction; p21WAF1/Cip1; MEK/ERK1/2; signal transduction; EPIDERMAL-GROWTH-FACTOR; PROTEIN-KINASE-C; P21(WAF1/CIP1) EXPRESSION; IMMUNE EVASION; PATHWAY; TRANSCRIPTION; POXVIRUS; P53; PHOSPHORYLATION; MULTIPLICATION;

D O I：

10.1590/S0074-02762010000300005

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

In this paper, we provide evidence that both the mRNA and protein levels of the cyclin-dependent kinase (CDK) inhibitor p21WAF1/CDK-interacting protein 1 (Cip1) increase upon infection of A431 cells with Vaccinia virus (VACV). In addition, the VACV growth factor (VGF) seems to be required for the gene expression because infection carried out with the mutant virus VACV-VGF-revealed that this strain was unable to stimulate its transcription. Our findings are also consistent with the notion that the VGF-mediated change in p21WAF1/Cip1 expression is dependent on tyrosine kinase pathway(s) and is partially dependent on mitogen-activated protein kinase/extracellular-signal regulated kinase 1/2. We believe that these pathways are biologically significant because VACV replication and dissemination was drastically affected when the infection was carried out in the presence of the relevant pharmacological inhibitors.

引用

页码：269 / 277

页数：9

共 40 条

[1] p21WAF1 expression by an activator of protein kinase C is regulated mainly at the post-transcriptional level in cells lacking p53:: important role of RNA stabilization [J].

Akashi, M ;

Osawa, Y ;

Koeffler, HP ;

Hachiya, M .

BIOCHEMICAL JOURNAL, 1999, 337 :607-616

[2] The vaccinia virus-stimulated mitogen-activated protein kinase (MAPK) pathway is required for virus multiplication [J].

Andrade, AA ;

Silva, PNG ;

Pereira, ACTC ;

De Sousa, LP ;

Ferreira, PCP ;

Gazzinelli, RT ;

Kroon, EG ;

Ropert, C ;

Bonjardim, CA .

BIOCHEMICAL JOURNAL, 2004, 381 :437-446

[3] EPIDERMAL GROWTH-FACTOR INHIBITS GROWTH OF A431 HUMAN EPIDERMOID CARCINOMA IN SERUM-FREE CELL-CULTURE [J].

BARNES, DW .

JOURNAL OF CELL BIOLOGY, 1982, 93 (01) :1-4

[4] VACCINIA VIRUS ENCODES A POLYPEPTIDE HOMOLOGOUS TO EPIDERMAL GROWTH-FACTOR AND TRANSFORMING GROWTH-FACTOR [J].

BROWN, JP ;

TWARDZIK, DR ;

MARQUARDT, H ;

TODARO, GJ .

NATURE, 1985, 313 (6002) :491-492

[5] Vaccinia virus transcription [J].

Broyles, SS .

JOURNAL OF GENERAL VIROLOGY, 2003, 84 :2293-2303

[6] DECREASED VIRULENCE OF RECOMBINANT VACCINIA VIRUS EXPRESSION VECTORS IS ASSOCIATED WITH A THYMIDINE KINASE-NEGATIVE PHENOTYPE [J].

BULLER, RML ;

SMITH, GL ;

CREMER, K ;

NOTKINS, AL ;

MOSS, B .

NATURE, 1985, 317 (6040) :813-815

[7] CELL PROLIFERATIVE RESPONSE TO VACCINIA VIRUS IS MEDIATED BY VGF [J].

BULLER, RML ;

CHAKRABARTI, S ;

MOSS, B ;

FREDRICKSON, T .

VIROLOGY, 1988, 164 (01) :182-192

[8]

BURNET FM, 1936, SPEC REP SER MED RES, V220, P555

[9] P53 functional impairment and high p21(waf1/cip1) expression in human T-cell lymphotropic/leukemia virus type I-transformed T cells [J].

Cereseto, A ;

Diella, F ;

Mulloy, JC ;

Cara, A ;

Michieli, P ;

Grassmann, R ;

Franchini, G ;

Klotman, ME .

BLOOD, 1996, 88 (05) :1551-1560

[10] Cell growth arrest and induction of cyclin-dependent kinase inhibitor p21(WAF1/CIP1) mediated by STAT1 [J].

Chin, YE ;

Kitagawa, M ;

Su, WCS ;

You, ZH ;

Iwamoto, Y ;

Fu, XY .

SCIENCE, 1996, 272 (5262) :719-722

← 1 2 3 4 →