High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe

被引：48
作者：
Aguirre, Aaron D. [1 ,2 ]
Sawinski, Juergen [3 ]
Huang, Shu-Wei [1 ,2 ]
Zhou, Chao [1 ,2 ]
Denk, Winfried [3 ]
Fujimoto, James G. [1 ,2 ]
机构：
[1] MIT, Elect Res Lab, Cambridge, MA 02139 USA
[2] MIT, Dept Elect Engn & Comp Sci, Cambridge, MA 02139 USA
[3] Max Planck Inst Med Res, D-69120 Heidelberg, Germany
来源：
OPTICS EXPRESS | 2010年 / 18卷 / 05期
基金：
美国国家卫生研究院;
关键词：
MEMS SCANNING CATHETER; REAL-TIME; CONFOCAL MICROSCOPE; HUMAN SKIN; TOMOGRAPHY; PHASE; LASER; DELAY; LINE; OCT;
D O I：
10.1364/OE.18.004222
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Optical coherence microscopy (OCM) is a promising technique for high resolution cellular imaging in human tissues. An OCM system for high-speed en face cellular resolution imaging was developed at 1060 nm wavelength at frame rates up to 5 Hz with resolutions of < 4 mu m axial and < 2 mu m transverse. The system utilized a novel polarization compensation method to combat wavelength dependent source polarization and achieve broadband electro-optic phase modulation compatible with ultrahigh axial resolution. In addition, the system incorporated an auto-focusing feature that enables precise, near real-time alignment of the confocal and coherence gates in tissue, allowing user-friendly optimization of image quality during the imaging procedure. Ex vivo cellular images of human esophagus, colon, and cervix as well as in vivo results from human skin are presented. Finally, the system design is demonstrated with a miniaturized piezoelectric fiber-scanning probe which can be adapted for laparoscopic and endoscopic imaging applications. (C) 2010 Optical Society of America
引用
收藏
页码：4222 / 4239
页数：18
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