Differential effect of H1 variant overproduction on gene expression is due to differences in the central globular domain

被引：35

作者：

Brown, DT ^{[1
]}

Gunjan, A ^{[1
]}

Alexander, BT ^{[1
]}

Sittman, DB ^{[1
]}

机构：

[1] Univ Mississippi, Med Ctr, Dept Biochem, Jackson, MS 39216 USA

来源：

NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 24期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1093/nar/25.24.5003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The in vivo overproduction of two mouse histone H1 variants in homologous mouse fibroblasts has opposite effects on gene expression. Overproduction of H1(0) results in repression of transcript levels of ail polymerase II genes tested. In contrast, overproduction of H1c results in elevated revels of transcripts. We created a series of chimeric H1 genes in which the regions encoding the three structural domains common to this family of these proteins were systematically switched. Overexpression of these genes in vivo resulted in the accumulation of large amounts of the chimeric H1 in chromatin. Analysis of the effects of overproduction of these proteins revealed that the differential effect of H1 variant overproduction on gene expression is due to differences in the central globular domain.

引用

页码：5003 / 5009

页数：7

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