Differential effect of H1 variant overproduction on gene expression is due to differences in the central globular domain

被引：35

作者：

Brown, DT ^{[1
]}

Gunjan, A ^{[1
]}

Alexander, BT ^{[1
]}

Sittman, DB ^{[1
]}

机构：

[1] Univ Mississippi, Med Ctr, Dept Biochem, Jackson, MS 39216 USA

来源：

NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 24期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1093/nar/25.24.5003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The in vivo overproduction of two mouse histone H1 variants in homologous mouse fibroblasts has opposite effects on gene expression. Overproduction of H1(0) results in repression of transcript levels of ail polymerase II genes tested. In contrast, overproduction of H1c results in elevated revels of transcripts. We created a series of chimeric H1 genes in which the regions encoding the three structural domains common to this family of these proteins were systematically switched. Overexpression of these genes in vivo resulted in the accumulation of large amounts of the chimeric H1 in chromatin. Analysis of the effects of overproduction of these proteins revealed that the differential effect of H1 variant overproduction on gene expression is due to differences in the central globular domain.

引用

页码：5003 / 5009

页数：7

共 76 条

[1] REGULATION OF HISTONE AND BETA-A-GLOBIN GENE-EXPRESSION DURING DIFFERENTIATION OF CHICKEN ERYTHROID-CELLS [J].

AFFOLTER, M ;

COTE, J ;

RENAUD, J ;

RUIZCARRILLO, A .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (10) :3663-3672

[2] ROLES OF H-1 DOMAINS IN DETERMINING HIGHER-ORDER CHROMATIN STRUCTURE AND H-1 LOCATION [J].

ALLAN, J ;

MITCHELL, T ;

HARBORNE, N ;

BOHM, L ;

CRANEROBINSON, C .

JOURNAL OF MOLECULAR BIOLOGY, 1986, 187 (04) :591-601

[3] EARLY INCREASE IN HISTONE-H1-DEGREES MESSENGER-RNA DURING DIFFERENTIATION OF F9 CELLS TO PARIETAL ENDODERM [J].

ALONSO, A ;

BREUER, B ;

BOUTERFA, H ;

DOENECKE, D .

EMBO JOURNAL, 1988, 7 (10) :3003-3008

[4] COMPARISON OF 3 ACTIN-CODING SEQUENCES IN THE MOUSE - EVOLUTIONARY RELATIONSHIPS BETWEEN THE ACTIN GENES OF WARM-BLOODED VERTEBRATES [J].

ALONSO, S ;

MINTY, A ;

BOURLET, Y ;

BUCKINGHAM, M .

JOURNAL OF MOLECULAR EVOLUTION, 1986, 23 (01) :11-22

[5] CONFORMATION OF HISTONE-H5 - ISOLATION AND CHARACTERIZATION OF GLOBULAR SEGMENT [J].

AVILES, FJ ;

CHAPMAN, GE ;

KNEALE, GG ;

CRANEROBINSON, C ;

BRADBURY, EM .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1978, 88 (02) :363-371

[6] SPECIFIC REGULATION OF XENOPUS CHROMOSOMAL 5S RIBOSOMAL-RNA GENE-TRANSCRIPTION IN-VIVO BY HISTONE H1 [J].

BOUVET, P ;

DIMITROV, S ;

WOLFFE, AP .

GENES & DEVELOPMENT, 1994, 8 (10) :1147-1159

[7] THE WINGED-HELIX DNA-BINDING MOTIF - ANOTHER HELIX-TURN-HELIX TAKEOFF [J].

BRENNAN, RG .

CELL, 1993, 74 (05) :773-776

[8] THE ROLE OF STABLE COMPLEXES THAT REPRESS AND ACTIVATE EUKARYOTIC GENES [J].

BROWN, DD .

CELL, 1984, 37 (02) :359-365

[9] Differential effect of H1 variant overexpression on cell cycle progression and gene expression [J].

Brown, DT ;

Alexander, BT ;

Sittman, DB .

NUCLEIC ACIDS RESEARCH, 1996, 24 (03) :486-493

[10]

BROWN DT, 1993, J BIOL CHEM, V268, P713

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