Negri bodies are viral factories with properties of liquid organelles

被引：220

作者：

Nikolic, Jovan ^{[1
]}

Le Bars, Romain ^{[1
]}

Lama, Zoe ^{[1
]}

Scrima, Nathalie ^{[1
]}

Lagaudriere-Gesbert, Cecile ^{[1
]}

Gaudin, Yves ^{[1
]}

Blondel, Danielle ^{[1
]}

机构：

[1] Univ Paris Saclay, Univ Paris Sud, CNRS, Inst Integrat Biol Cell I2BC,CEA, F-91198 Gif Sur Yvette, France

来源：

NATURE COMMUNICATIONS | 2017年 / 8卷

关键词：

VIRUS-INFECTED CELLS; INCLUSION-BODY FORMATION; RABIES VIRUS; PHOSPHOPROTEIN P; PHASE-SEPARATION; IN-VIVO; PROTEIN; REPLICATION; RNA; NUCLEOPROTEIN;

D O I：

10.1038/s41467-017-00102-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Replication of Mononegavirales occurs in viral factories which form inclusions in the host-cell cytoplasm. For rabies virus, those inclusions are called Negri bodies (NBs). We report that NBs have characteristics similar to those of liquid organelles: they are spherical, they fuse to form larger structures, and they disappear upon hypotonic shock. Their liquid phase is confirmed by FRAP experiments. Live-cell imaging indicates that viral nucleocapsids are ejected from NBs and transported along microtubules to form either new virions or secondary viral factories. Coexpression of rabies virus N and P proteins results in cytoplasmic inclusions recapitulating NBs properties. This minimal system reveals that an intrinsically disordered domain and the dimerization domain of P are essential for Negri bodies-like structures formation. We suggest that formation of liquid viral factories by phase separation is common among Mononegavirales and allows specific recruitment and concentration of viral proteins but also the escape to cellular antiviral response.

引用

页数：13

共 56 条

[1] Rabies Virus Transcription and Replication [J].