Altitudinal Distribution Patterns of Phyllosphere Microbial Communities and Their Contribution to Silage Fermentation of Kobresia pygmaea Along the Elevation Gradient on the Tibetan Plateau

被引：5

作者：

Yang, Xin ^{[1
]}

Bao, Yuhong ^{[2
,3
]}

Shao, Tao ^{[1
]}

Wang, Wenkang ^{[1
]}

Ma, Pengfei ^{[1
]}

Wang, Wenbo ^{[1
]}

Gallo, Antonio ^{[4
]}

Yuan, Xianjun ^{[1
]}

机构：

[1] Nanjing Agr Univ, Inst Ensiling & Proc Grass, Coll Agrograssland Sci, Nanjing, Peoples R China

[2] State Key Lab Germplasm Resources & Genet Improvem, Lhasa, Peoples R China

[3] TAR Acad Agr & Anim Husb Sci, Inst Grass Sci, Lhasa, Peoples R China

[4] Univ Cattolica Sacro Cuore, Fac Agr Food & Environml Sci, Dept Anim Sci Food & Nutr DIANA, Piacenza, Italy

来源：

FRONTIERS IN MICROBIOLOGY | 2022年 / 13卷

基金：

中国国家自然科学基金;

关键词：

elevational gradients; Kobresia pygmaea; fermentation quality; Tibetan Plateau; bacterial diversity; fungal diversity;

D O I：

10.3389/fmicb.2022.874582

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The study aimed to reveal altitudinal distribution patterns of phyllosphere microbial communities and silage fermentation of Kobresia pygmaea along the elevation gradient on the Tibetan Plateau. The K. pygmaea was individually collected from 2,500, 3,000, 4,000, 4,500, and 5,000 m above sea level (a.s.l.) on the Tibetan Plateau and ensiled for 60 days, respectively. The phyllosphere bacterial diversity increased while fungal diversity decreased along the elevation gradient, and bacterial and fungal richness showed a unimodal distribution with peak abundance at 4,000 and 3,000 m a.s.l., respectively. After 60 days of ensiling, the bacterial and fungal community composition changed but did not exhibit clear altitudinal distribution patterns. All K. pygmaea underwent a weak fermentation indicated by pH above 5.0 and low ratio of lactic/acetic acid (LA/AA). The S5000 and S3000 showed the highest and lowest pH, respectively. Although Lactobacillus dominated S4000 after 60 days of ensiling, S4000 still exhibited poor fermentation quality as well as silages from the other four regions. The higher ammonia N concentrations in S3000 and S4000 than the other silages were consistent with the detectable butyric acid in S3000 and S4000. The silage fermentation of K. pygmaea collected from five regions exhibited poor fermentation quality, thereby inoculating lactic acid bacteria to K. pygmaea before ensiling is highly recommended to improve fermentation quality on the Tibetan Plateau.

引用

页数：12