Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types

被引：29

作者：

Tarakanchikova, Yana ^{[1
,2
,3
,4
]}

Alzubi, Jamal ^{[5
,6
]}

Pennucci, Valentina ^{[5
,6
]}

Follo, Marie ^{[7
]}

Kochergin, Boris ^{[1
,8
]}

Muslimov, Albert ^{[3
]}

Skovorodkin, Ilya ^{[9
]}

Vainio, Seppo ^{[9
]}

Antipina, Maria N. ^{[10
]}

Atkin, Vsevolod ^{[11
]}

Popov, Alexey ^{[2
]}

Meglinski, Igor ^{[2
,12
,13
]}

Cathomen, Toni ^{[5
,6
]}

Cornu, Tatjana, I ^{[5
,6
]}

Gorin, Dmitry A. ^{[14
]}

Sukhorukov, Gleb B. ^{[14
,15
]}

Nazarenko, Irina ^{[1
,16
,17
]}

机构：

[1] Univ Freiburg, Fac Med, Med Ctr, Inst Infect Prevent & Hosp Epidemiol, D-79106 Freiburg, Germany

[2] Univ Oulu, Optoelect & Measurement Tech Res Unit, POB 4500, Oulu 90014, Finland

[3] St Petersburg Acad Univ, Nanobiotechnol Lab, St Petersburg 194021, Russia

[4] Peter Great St Petersburg Polytech Univ, RASA Ctr St Petersburg, St Petersburg 195251, Russia

[5] Univ Freiburg, Fac Med, Med Ctr, Inst Transfus Med & Gene Therapy, D-79106 Freiburg, Germany

[6] Univ Freiburg, Fac Med, Med Ctr, Ctr Chron Immunodeficiency, D-79106 Freiburg, Germany

[7] Univ Freiburg, Fac Med, Med Ctr, Dept Med 1, D-153000 Freiburg, Germany

[8] Ivanovo State Univ Chem & Technol, Dept Inorgan Chem, Sheremetievskiy Ave 7, Ivanovo 153000, Russia

[9] Univ Oulu, Infotech Oulu, Lab Dev Biol, Fac Biochem & Mol Med,Bioctr Oulu,Borealis Bioban, Oulu 138634, Finland

[10] ASTAR, Inst Mat Res & Engn, Singapore 138634, Singapore

[11] Saratov NG Chernyshevskii State Univ, Educ Res Inst Nanostruct & Biosyst, Astrakhanskaya 83, Saratov 410012, Russia

[12] Aston Univ, Sch Engn & Appl Sci, Aston Inst Mat Res, Birmingham B4 7ET, W Midlands, England

[13] Aston Univ, Sch Life & Hlth Sci, Birmingham B4 7ET, W Midlands, England

[14] Skolkovo Innovat Ctr, Skolkovo Inst Sci & Technol, Skoltech Ctr Photon & Quantum Mat, Bldg 3, Moscow 143026, Russia

[15] Queen Mary Univ London, Sch Engn & Mat Sci, London B4 7ET, England

[16] German Canc Consortium DKTK, Partner Site Freiburg, B47ET, Heidelberg, Germany

[17] German Canc Res Ctr, B47ET, Heidelberg, Germany

来源：

SMALL | 2020年 / 16卷 / 03期

关键词：

biomimetics; drug delivery; extracellular vesicles; gene delivery; microcapsules; nanocapsules; HEMATOPOIETIC PROGENITOR CELLS; MULTILAYER CAPSULES; STEM-CELLS; CALCIUM-CARBONATE; SIRNA; MICROCAPSULES; EXOSOMES; ENCAPSULATION; PARTICLES; CARRIERS;

D O I：

10.1002/smll.201904880

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Efficient delivery of genetic material to primary cells remains challenging. Here, efficient transfer of genetic material is presented using synthetic biodegradable nanocarriers, resembling extracellular vesicles in their biomechanical properties. This is based on two main technological achievements: generation of soft biodegradable polyelectrolyte capsules in nanosize and efficient application of the nanocapsules for co-transfer of different RNAs to tumor cell lines and primary cells, including hematopoietic progenitor cells and primary T cells. Near to 100% efficiency is reached using only 2.5 x 10(-4) pmol of siRNA, and 1 x 10(-3) nmol of mRNA per cell, which is several magnitude orders below the amounts reported for any of methods published so far. The data show that biodegradable nanocapsules represent a universal and highly efficient biomimetic platform for the transfer of genetic material with the utmost potential to revolutionize gene transfer technology in vitro and in vivo.

引用

页数：10

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