Engineering a chimeric malate two-component system by introducing a positive feedback loop in Escherichia coli

被引：0

作者：

Ganesh, Irisappan ^{[1
]}

Maruthamuthu, Murali Kannan ^{[1
]}

Hong, Soon Ho ^{[1
]}

机构：

[1] Univ Ulsan, Dept Chem Engn, Ulsan 44610, South Korea

来源：

KOREAN JOURNAL OF CHEMICAL ENGINEERING | 2016年 / 33卷 / 03期

基金：

新加坡国家研究基金会;

关键词：

Escherichia coli; Malate; Positive Feedback Loop; Two-component System; EXPRESSION;

D O I：

10.1007/s11814-015-0209-5

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Previous studies constructed a chimeric MalKZ two-component system to sense environmental malate. In this study, we used a positive feedback loop to accelerate and amplify the output signal indicating malate concentration. The positive feedback loop was constructed by cloning ompR gene, which encodes ompC and induces OmpR protein; ompC promoter was used to control the process. The transcriptional expression profile showed that the expression level of ompC gene increased about two-fold after the positive feedback loop was introduced. When GFP was used as a reporter protein, a 71% increase in fluorescence level was observed. The results indicate that the signal transduction kinetics of MalKZ can be engineered by introducing the positive feedback loop.

引用

页码：972 / 975

页数：4

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