In vivo expression of Toll-like receptor 2, Toll-like receptor 4, CSF2 and LY64 in Chinese chronic periodontitis patients

被引:24
作者
Sun, Y.
Guo, Q-M
Liu, D-L
Zhang, M-Z
Shu, R. [1 ]
机构
[1] Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9, Dept Periodontol, Shanghai 200011, Peoples R China
关键词
Toll-like receptor 2; Toll-like receptor 4; colony-stimulating factor 2; LY64; chronic periodontitis; HUMAN GINGIVAL FIBROBLASTS; MEMBRANE-BOUND CD14; EPITHELIAL-CELLS; GM-CSF; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; PORPHYROMONAS-GINGIVALIS; ALVEOLAR MACROPHAGES; ENDOTOXIN TOLERANCE; KINASE EXPRESSION; GAMMA-INTERFERON;
D O I
10.1111/j.1601-0825.2009.01630.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Objective: Toll-like receptors (TLRs) are the essential components in the innate and adaptive immune systems. Colony stimulating factor 2 (CSF2) is a cytokine that may prevent endotoxin tolerance, and LY64 has the ability to interfere with the recognition of bacteria via TLR4. The aim of this study was to explore the in vivo expressions of TLR2, TLR4, CSF2 and LY64 in Chinese chronic periodontitis patients. Methods: Gingival biopsies were collected from 24 chronic periodontitis patients and 19 healthy controls. The gene expression profiles of TLR2, TLR4, CSF2 and LY64 were investigated by real-time polymerase chain reaction, and the protein expressions of TLR2 and TLR4 were detected by immunohistochemistry. In addition, the levels of CSF2 in gingival crevicular fluid (GCF) were determined by ELISA. Results: The higher mRNA expressions of TLR2, TLR4 and CSF2, and the lower mRNA expression of LY64 were detected in chronic periodontitis patients. And the increased protein expressions of TLR2 and TLR4 were confirmed by immunohistochemistry. In addition, the increase of total amount of CSF2 in GCF was observed in chronic periodontitis patients. Conclusions: Our results suggest that TLR2 and TLR4 may play a role in periodontal pathogenesis. In addition, CSF2 and LY64 may contribute to the regulation of inflammatory response and maintaining periodontal homeostasis.
引用
收藏
页码:343 / 350
页数:8
相关论文
共 41 条
[1]   Gamma interferon and granulocyte/monocyte colony-stimulating factor prevent endotoxin tolerance in human monocytes by promoting interleukin-1 receptor-associated kinase expression and its association to MyD88 and not by modulating TLR4 expression [J].
Adib-Conquy, M ;
Cavaillon, JM .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (31) :27927-27934
[2]   Bacterial fimbriae and their peptides activate human gingival epithelial cells through toll-like receptor 2 [J].
Asai, Y ;
Ohyama, Y ;
Gen, K ;
Ogawa, T .
INFECTION AND IMMUNITY, 2001, 69 (12) :7387-7395
[3]   Biological markers for evaluation of root resorption [J].
Balducci, Laura ;
Ramachandran, Amsaveni ;
Hao, Jianjun ;
Narayanan, Karthikeyan ;
Evans, Carla ;
George, Anne .
ARCHIVES OF ORAL BIOLOGY, 2007, 52 (03) :203-208
[4]  
Bergstrom Jan, 2004, Odontology, V92, P1
[5]   Age-dependent decrease in Toll-like receptor 4-mediated proinflammatory cytokine production and mitogen-activated protein kinase expression [J].
Boehmer, ED ;
Goral, J ;
Faunce, DE ;
Kovacs, EJ .
JOURNAL OF LEUKOCYTE BIOLOGY, 2004, 75 (02) :342-349
[6]  
Bulkacz J, 2002, CARRANZAS CLIN PERIO, P254
[7]   Tobacco smoking inhibits expression of proinflammatory Cytokines and activation of IL-1R-Associated kinase, p38, and NF-κB in alveolar macrophages stimulated with TLR2 and TLR4 Agonists [J].
Chen, Haiyan ;
Cowan, Mark J. ;
Hasday, Jeffrey D. ;
Vogel, Stefanie N. ;
Medvedev, Andrei E. .
JOURNAL OF IMMUNOLOGY, 2007, 179 (09) :6097-6106
[8]   Negative regulation of Toll-like receptor 4 signaling by the Toll-like receptor homolog RP105 [J].
Divanovic, S ;
Trompette, A ;
Atabani, SF ;
Madan, R ;
Golenbock, DT ;
Visintin, A ;
Finberg, RW ;
Tarakhovsky, A ;
Vogel, SN ;
Belkaid, Y ;
Kurt-Jones, EA ;
Karp, CL .
NATURE IMMUNOLOGY, 2005, 6 (06) :571-578
[9]   Toll-like receptor 2 expression is decreased on alveolar macrophages in cigarette smokers and COPD patients [J].
Droemann, D ;
Goldmann, T ;
Tiedje, T ;
Zabel, P ;
Dalhoff, K ;
Schaaf, B .
RESPIRATORY RESEARCH, 2005, 6 (1)
[10]   Delayed neutrophil apoptosis in chronic periodontitis patients [J].
Gamonal, J ;
Sanz, M ;
O'Connor, A ;
Acevedo, A ;
Suarez, I ;
Sanz, A ;
Martínez, B ;
Silva, A .
JOURNAL OF CLINICAL PERIODONTOLOGY, 2003, 30 (07) :616-623