Recent Advances in the Use of Molecular Methods for the Diagnosis of Bacterial Infections

被引:28
作者
Gerace, Elisabetta [1 ]
Mancuso, Giuseppe [2 ]
Midiri, Angelina [2 ]
Poidomani, Stefano [2 ]
Zummo, Sebastiana [2 ]
Biondo, Carmelo [2 ]
机构
[1] ASP Azienda Sanit Provinciale, I-90141 Palermo, Italy
[2] Univ Messina, Dept Human Pathol, I-98125 Messina, Italy
来源
PATHOGENS | 2022年 / 11卷 / 06期
关键词
multidrug-resistant bacteria; antimicrobial resistance; ESKAPE; genotyping methods; ISOTHERMAL AMPLIFICATION LAMP; SPECTRUM BETA-LACTAMASE; REAL-TIME PCR; RAPID DETECTION; ANTIMICROBIAL RESISTANCE; DNA MICROARRAY; PATHOGENS; GENES; PERSPECTIVES; VANCOMYCIN;
D O I
10.3390/pathogens11060663
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Infections caused by bacteria have a major impact on public health-related morbidity and mortality. Despite major advances in the prevention and treatment of bacterial infections, the latter continue to represent a significant economic and social burden worldwide. The WHO compiled a list of six highly virulent multidrug-resistant bacteria named ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species) responsible for life-threatening diseases. Taken together with Clostridioides difficile, Escherichia coli, Campylobacter spp., (C. jejuni and C. coli), Legionella spp., Salmonella spp., and Neisseria gonorrhoeae, all of these microorganisms are the leading causes of nosocomial infections. The rapid and accurate detection of these pathogens is not only important for the early initiation of appropriate antibiotic therapy, but also for resolving outbreaks and minimizing subsequent antimicrobial resistance. The need for ever-improving molecular diagnostic techniques is also of fundamental importance for improving epidemiological surveillance of bacterial infections. In this review, we aim to discuss the recent advances on the use of molecular techniques based on genomic and proteomic approaches for the diagnosis of bacterial infections. The advantages and limitations of each of the techniques considered are also discussed.
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页数:13
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