14-3-3ζ Reduces DNA Damage by Interacting With and Stabilizing Proliferating Cell Nuclear Antigen

被引：23

作者：

Gao, Xuejuan ^{[1
]}

Dan, Songsong ^{[1
]}

Xie, Yingying ^{[1
]}

Qin, Huanhuan ^{[1
]}

Tang, Donge ^{[1
]}

Liu, Xiaohui ^{[1
]}

He, Qing-Yu ^{[1
]}

Liu, Langxia ^{[1
]}

机构：

[1] Jinan Univ, Inst Life & Hlth Engn, Key Lab Funct Prot Res, Guangdong Higher Educ Inst, Guangzhou 510632, Guangdong, Peoples R China

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 2015年 / 116卷 / 01期

基金：

中国国家自然科学基金;

关键词：

14-3-3; PCNA; PROTEIN-PROTEIN INTERACTION; STABILITY; DNA DAMAGE; LUNG-CANCER CELLS; TRANSLESION SYNTHESIS; PROTEOMIC ANALYSIS; STRUCTURAL BASIS; IN-VIVO; PROTEINS; PCNA; 14-3-3-PROTEINS; IDENTIFICATION; REPLICATION;

D O I：

10.1002/jcb.24955

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proliferating cell nuclear antigen (PCNA) is a processivity factor of DNA replication which plays critical roles in the regulation of DNA replication and repair. In this study, we show that PCNA interacts directly in vitro and in cells with 14-3-3, an adaptor protein that regulates cell growth and response to DNA damage in eukaryotes. The interaction is mediated by at least two PCNA-binding sites on 14-3-3, one of which is a novel non-canonical PIP (PCNA interacting protein) box. We find that DNA damages induced by UVC irradiation and MMS (methyl methanesulfonate) can enhance both the interaction of these two proteins and their co-localization with chromatin. Functional analyses suggest that 14-3-3 stabilizes PCNA possibly by regulating its ubiquitination, which impacts on DNA damage repair and cell viability. J. Cell. Biochem. 116: 158-169, 2015. (c) 2014 Wiley Periodicals, Inc.

引用

页码：158 / 169

页数：12

共 50 条

[1] 14-3-3 proteins: A historic overview [J].

Aitken, Alastair .

SEMINARS IN CANCER BIOLOGY, 2006, 16 (03) :162-172

[2] Sequential assembly of translesion DNA polymerases at UV-induced DNA damage sites [J].

Andersen, Parker L. ;

Xu, Fang ;

Ziola, Barry ;

McGregor, W. Glen ;

Xiao, Wei .

MOLECULAR BIOLOGY OF THE CELL, 2011, 22 (13) :2373-2383

[3] Identification of differentially expressed genes in oral squamous cell carcinoma [J].

Arora, S ;

Matta, A ;

Shukla, NK ;

Deo, SVS ;

Ralhan, R .

MOLECULAR CARCINOGENESIS, 2005, 42 (02) :97-108

[4] Ubiquitylation of the 9-1-1 Checkpoint Clamp Is Independent of Rad6-Rad18 and DNA Damage [J].

Davies, Adelina A. ;

Neiss, Andrea ;

Ulrich, Helle D. .

CELL, 2010, 141 (06) :1080-1087

[5]

Dieckman Lynne M, 2012, Subcell Biochem, V62, P281, DOI 10.1007/978-94-007-4572-8_15

[6] Unlocking the code of 14-3-3 [J].

Dougherty, MK ;

Morrison, DK .

JOURNAL OF CELL SCIENCE, 2004, 117 (10) :1875-1884

[7] Biochemical characterization of DNA damage checkpoint complexes:: Clamp loader and clamp complexes with specificity for 5′ recessed DNA [J].

Ellison, V ;

Stillman, B .

PLOS BIOLOGY, 2003, 1 (02) :231-243

[8] Nuclear dynamics of PCNA in DNA replication and repair [J].

Essers, J ;

Theil, AF ;

Baldeyron, C ;

van Cappellen, WA ;

Houtsmuller, AB ;

Kanaar, R ;

Vermeulen, W .

MOLECULAR AND CELLULAR BIOLOGY, 2005, 25 (21) :9350-9359

[9] Up-regulation of 14-3-3ζ in lung cancer and its implication as prognostic and therapeutic target [J].

Fan, Tao ;

Li, Ruiyun ;

Todd, Nevins W. ;

Qiu, Qi ;

Fang, Hong-Bin ;

Wang, Huijun ;

Shen, Jianjun ;

Zhao, Richard Y. ;

Caraway, Nancy P. ;

Katz, Ruth L. ;

Stass, Sanford A. ;

Jiang, Feng .

CANCER RESEARCH, 2007, 67 (16) :7901-7906

[10] Identification and analysis of glycogen synthase kinase 3 beta1 interactome [J].

Gao, Xuejuan ;

Wang, Jian-Ying ;

Gao, Ling-Mei ;

Yin, Xing-Feng ;

Liu, Langxia .

CELL BIOLOGY INTERNATIONAL, 2013, 37 (08) :768-779

← 1 2 3 4 5 →