Role of External Loops of Human Ceruloplasmin in Copper Loading by ATP7B and Ccc2p

被引：12

作者：

Maio, Nunziata

Polticelli, Fabio

De Francesco, Giovanni ^{[1
]}

Rizzo, Gianluca

di Patti, Maria Carmela Bonaccorsi ^{[1
]}

Musci, Giovanni ^{[2
,3
]}

机构：

[1] Univ Roma La Sapienza, Dept Sci Biochim, I-00185 Rome, Italy

[2] Univ ROMA TRE, Dipartimento Biol, I-00146 Rome, Italy

[3] Univ Molise, Dipartimento Sci & Tecnol Agroalimentari, I-86100 Campobasso, Italy

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2010年 / 285卷 / 27期

关键词：

PICHIA-PASTORIS; ACERULOPLASMINEMIA; EXPRESSION; DISORDER; ATPASES; PROTEIN; SYSTEM;

D O I：

10.1074/jbc.M109.090027

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ceruloplasmin is a multicopper oxidase required for correct iron homeostasis. Previously, we have identified a ceruloplasmin mutant associated with the iron overload disease aceruloplasminemia, which was unable to acquire copper from themammalian pump ATP7B but could be produced in an enzymatically active form in yeast. Here, we report the expression of recombinant ceruloplasmin in the yeast Pichia pastoris and the study of the role of five surface-exposed loops in copper incorporation by comparing the efficiencies of mammalian ATP7B and yeast Ccc2p. The possibility to "mix and match" mammalian and yeast multicopper oxidases and copper ATPases can provide clues on the molecular features underlying the process of copper loading in multicopper oxidases.

引用

收藏

页码：20507 / 20513

页数：7

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