Multiple Locations of Peptides in the Hydrocarbon Core of Gel-Phase Membranes Revealed by Peptide 13C to Lipid 2H Rotational-Echo Double-Resonance Solid-State Nuclear Magnetic Resonance

Membrane locations of peptides and proteins are often critical to their functions. Solid-state rotational-echo double-resonance (REDOR) nuclear magnetic resonance is applied to probe the locations of two peptides via peptide (CO)-C-13 to lipid H-2 distance measurements. The peptides are KALP, an alpha-helical membrane-spanning peptide, and HFP, the beta-sheet N-terminal fusion peptide of the HIV gp41 fusion protein that plays an important role in HIV-host cell membrane fusion. Both peptides are shown to have at least two distinct locations within the hydrocarbon core of gel-phase membranes. The multiple locations are attributed to snorkeling of lysine side chains for KALP and to the distribution of antiparallel beta-sheet registries for HFP. The relative population of each location is also quantitated. To the best of our knowledge, this is the first clear experimental support of multiple peptide locations within the membrane hydrocarbon core. These data are for gel-phase membranes, but the approach should work for liquid-ordered membranes containing cholesterol and may be applicable to liquid-disordered membranes with appropriate additional analysis to take into account protein and lipid motion. This paper also describes the methodological development of (CO)-C-13-H-2 REDOR using the lyophilized I4 peptide that is alpha-helical and (CO)-C-13-labeled at A9 and 2H(alpha)-labeled at A8. The I4 spins are well-approximated as an ensemble of isolated (CO)-C-13-H-2 spin pairs each separated by 5.0 angstrom with a 37 Hz dipolar coupling. A pulse sequence with rectangular 100 kHz H-2 pi pulses results in rapid and extensive buildup of REDOR (Delta S/S-0) with a dephasing time (tau). The buildup is well-fit by a simple exponential function with a rate of 24 Hz and an extent close to 1. These parameter values reflect nonradiative transitions between the 2H spin states during the dephasing period. Each spin pair spends approximately two-thirds of its time in the (CO)-C-13-H-2 (m = +/-1) states and approximately one-third of its time in the (CO)-C-13-H-2 (m = 0) state and contributes to the Delta S/S-0 buildup during the former but not the latter time segments.