Shielding of the Geomagnetic Field Alters Actin Assembly and Inhibits Cell Motility in Human Neuroblastoma Cells

被引:36
|
作者
Mo, Wei-Chuan [1 ,2 ]
Zhang, Zi-Jian [1 ,3 ]
Wang, Dong-Liang [1 ]
Liu, Ying [1 ]
Bartlett, Perry F. [2 ]
He, Rong-Qiao [1 ,4 ,5 ]
机构
[1] Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Cognit Sci, Beijing 100101, Peoples R China
[2] Univ Queensland, Queensland Brain Inst, Brisbane, Qld 4072, Australia
[3] Beijing Univ Chinese Med, Beijing 100029, Peoples R China
[4] Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China
[5] Capital Med Univ, Beijing Inst Brain Disorders, Alzheimers Dis Ctr, Beijing 10069, Peoples R China
来源
SCIENTIFIC REPORTS | 2016年 / 6卷
关键词
ZERO MAGNETIC-FIELD; HYPOMAGNETIC FIELD; F-ACTIN; ADHESION; GROWTH; EXPOSURE; MODEL; MAGNETORECEPTION; CRYPTOCHROME; CYTOSKELETON;
D O I
10.1038/srep22624
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Accumulating evidence has shown that absence of the geomagnetic field (GMF), the so-called hypomagnetic field (HMF) environment, alters the biological functions in seemingly nonmagnetosensitive cells and organisms, which indicates that the GMF could be sensed by non-ironrich and non-photo-sensing cells. The underlying mechanisms of the HMF effects on those cells are closely related to their GMF sensation but remain poorly understood so far. Previously, we found that the HMF represses expressions of genes associated with cell migration and cytoskeleton assembly in human neuroblastoma cells (SH-SY5Y cell line). Here, we measured the HMF-induced changes on cell morphology, adhesion, motility and actin cytoskeleton in SH-SY5Y cells. The HMF inhibited cell adhesion and migration accompanied with a reduction in cellular F-actin amount. Moreover, following exposure to the HMF, the number of cell processes was reduced and cells were smaller in size and more round in shape. Furthermore, disordered kinetics of actin assembly in vitro were observed during exposure to the HMF, as evidenced by the presence of granule and meshed products. These results indicate that elimination of the GMF affects assembly of the motility-related actin cytoskeleton, and suggest that F-actin is a target of HMF exposure and probably a mediator of GMF sensation.
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页数:15
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