Lipase catalyzed esterification of formic acid in solvent and solvent-free systems

被引：16

作者：

Aljawish, Abdulhadi ^{[1
]}

Heuson, Egon ^{[1
]}

Bigan, Muriel ^{[1
]}

Froidevaux, Renato ^{[1
]}

机构：

[1] Univ Lille, INRA, ISA, Univ Artois,Univ Littoral Cote Opale,EA 7394,ICV, F-59000 Lille, France

来源：

BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY | 2019年 / 20卷

关键词：

Esterification; Lipase; Formic acid; Glycerol; Solvent-free system; LACTIC-ACID; ENZYMATIC ESTERIFICATION; ESTERS; FORMATE; TRANSESTERIFICATION;

D O I：

10.1016/j.bcab.2019.101221

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Esterification reaction between formic acid and alcohols (C4, C8) catalyzed by lipases was performed in solvent and solvent-free systems. High Performance Liquid Chromatography (HPLC) was used to study the kinetic parameters (Michaelis-Menten) and the reaction conditions by monitoring the ester synthesis. The optimal conditions for formate ester synthesis were found to be: 0.5 M of formic acid, 1.5 M of alcohol, 2% of Novozym 435 at 40 degrees C and 400 rpm in acetonitrile solvent without molecular sieves. In solvent-free system, the optimal conditions were found to be: 1 M of formic acid, 10 M of alcohol, 2% of Novozym 435 at 40 degrees C and 400 rpm without molecular sieves. These optimal conditions resulted in maximum ester yield about 90% for 8 h in solvent system and 5 h in solvent-free system. Among all esterification reactions, the esterification reaction of formic acid with octan-1-ol in solvent-free system was the better regarding the quantity of esterified formic acid, catalytic efficiency of lipase and the required reaction time. Under these optimal conditions, the biocatalyst could be reused for six reaction cycles keeping around 97% of its initial activity after treatment process with n-hexane. Consequently, this enzymatic procedure in solvent-free system could provide formate esters for food and cosmetic applications.

引用

页数：10

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