Depleted histone deacetylase 3 or restored microRNA-19b-1-5p facilitates recovery of spinal cord injury via inactivating JAK2/STAT3 signaling pathway

被引：5

作者：

Niu, Honghua ^{[1
]}

Song, Wenchao ^{[1
]}

Pei, Dongyang ^{[1
]}

Ma, Chen ^{[1
]}

Liu, Fudong ^{[1
]}

Li, Yan ^{[1
]}

Han, Shuaiwei ^{[1
]}

机构：

[1] Henan Prov Orthoped Hosp, Luoyang Orthoped Traumatol Hosp Henan Prov, Microtraumat Orthoped, 100 Yongping Rd, Zhengzhou 450000, Henan, Peoples R China

来源：

GENOMICS | 2022年 / 114卷 / 02期

关键词：

Spinal cord injury; Histone deacetylase 3; MicroRNA-19b-1-5p; Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway; Astrocyte; HDAC3; ACTIVATION; GFAP;

D O I：

10.1016/j.ygeno.2021.12.019

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We intended to discuss the influence of histone deacetylase 3 (HDAC3) on spinal cord injury (SCI) by regulating microRNA-19b-1-5p (miR-19b-1-5p) and janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) pathway. In a rat model, the role of HDAC3 and miR-19b-1-5p in SCI was identified through detecting motor function, serum inflammation, pathological damage, cell apoptosis and GFAP expression. Also, by measuring GFAP expression and migration of spinal cord astrocytes, the effects of HDAC3 and miR-19b-1-5p in SCI were identified in vitro. Restoration of miR-19b-1-5p or depletion of HDAC3 alleviated motor function, inflammation, relieved pathological damage and reduced apoptosis, and reduced GFAP expression in the spinal cord tissue of SCI rats. Up-regulating miR-19b-1-5p or down-regulating HDAC3 decreased migration and GFAP expression of injured astrocytes. Our study presents that down-regulated HDAC3 can facilitate the recovery of SCI via inhibiting the activation of JAK2/STAT3 pathway by up-regulating miR-19b-1-5p.

引用

页数：9

共 42 条

[1] Diagnostic Value of Serum Levels of GFAP, pNF-H, and NSE Compared With Clinical Findings in Severity Assessment of Human Traumatic Spinal Cord Injury [J].

Ahadi, Reza ;

Khodagholi, Fariba ;

Daneshi, Abdolhadi ;

Vafaei, Ali ;

Mafi, Amir Ali ;

Jorjani, Masoumeh .

SPINE, 2015, 40 (14) :E823-E830

[2] AG490 ameliorates early brain injury via inhibition of JAK2/STAT3-mediated regulation of HMGB1 in subarachnoid hemorrhage [J].

An, Ji-Yang ;

Pang, Hong-Gang ;

Huang, Ting-Qin ;

Song, Jin-Ning ;

Li, Dan-Dong ;

Zhao, Yong-Lin ;

Ma, Xu-Dong .

EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2018, 15 (02) :1330-1338

[3] The Process and Strategy for Developing Selective Histone Deacetylase 3 Inhibitors [J].

Cao, Fangyuan ;

Zwinderman, Martijn R. H. ;

Dekker, Frank J. .

MOLECULES, 2018, 23 (03)

[4] Valproic acid attenuates traumatic spinal cord injury-induced inflammation via STAT1 and NF-κB pathway dependent of HDAC3 [J].

Chen, Shoubo ;

Ye, Jingfang ;

Chen, Xiangrong ;

Shi, Jinnan ;

Wu, Wenhua ;

Lin, Wenping ;

Lin, Weibin ;

Li, Yasong ;

Fu, Huangde ;

Li, Shun .

JOURNAL OF NEUROINFLAMMATION, 2018, 15

[5] Effects of long non-coding RNA LINC00667 on renal tubular epithelial cell proliferation, apoptosis and renal fibrosis via the miR-19b-3p/LINC00667/CTGF signaling pathway in chronic renal failure [J].

Chen, Wen ;

Zhou, Zhong-Qi ;

Ren, Yue-Qin ;

Zhang, Lei ;

Sun, Li-Na ;

Man, Yu-Lin ;

Wang, Zhi-Kui .

CELLULAR SIGNALLING, 2019, 54 :102-114

[6]

Dai J, 2018, EUR REV MED PHARMACO, V22, P6609, DOI 10.26355/eurrev_201810_16135

[7] MiR-19b-3p attenuates IL-1β induced extracellular matrix degradation and inflammatory injury in chondrocytes by targeting GRK6 [J].

Duan, Liang ;

Duan, Dapeng ;

Wei, Wenbo ;

Sun, Zhengming ;

Xu, Honghai ;

Guo, Lipeng ;

Wu, Xuanhui .

MOLECULAR AND CELLULAR BIOCHEMISTRY, 2019, 459 (1-2) :205-214

[8] HDAC3 mediates smoking-induced pancreatic cancer [J].

Edderkaoui, Mouad ;

Xu, Shiping ;

Chheda, Chintan ;

Morvaridi, Susan ;

Hu, Robert W. ;

Grippo, Paul J. ;

Mascarinas, Emman ;

Principe, Daniel R. ;

Knudsen, Beatrice ;

Xue, Jing ;

Habtezion, Aida ;

Uyeminami, Dale ;

Pinkerton, Kent E. ;

Pandol, Stephen J. .

ONCOTARGET, 2016, 7 (07) :7747-7760

[9] Integrative regulation of physiology by histone deacetylase 3 [J].

Emmett, Matthew J. ;

Lazar, Mitchell A. .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2019, 20 (02) :102-115

[10] The Combination of Adipose-derived Schwann-like Cells and Acellular Nerve Allografts Promotes Sciatic Nerve Regeneration and Repair through the JAK2/STAT3 Signaling Pathway in Rats [J].

Fu, Xiu-mei ;

Wang, Ying ;

Fu, Wen-liang ;

Liu, Dong-hui ;

Zhang, Cheng-yun ;

Wang, Qiao-ling ;

Tong, Xiao-jie .

NEUROSCIENCE, 2019, 422 :134-145

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