Drosophila Mcm10 interacts with members of the prereplication complex and is required for proper chromosome condensation

被引：69

作者：

Christensen, TW ^{[1
]}

Tye, BK ^{[1
]}

机构：

[1] Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA

来源：

MOLECULAR BIOLOGY OF THE CELL | 2003年 / 14卷 / 06期

关键词：

D O I：

10.1091/mbc.E02-11-0706

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Mcm10 is required for the initiation of DNA replication in Saccharomyces cerevisiae. We have cloned MCM10 from Drosophila melanogaster and show that it complements a ScMCM10 null mutant. Moreover, Mcm10 interacts with key members of the prereplication complex: Mcm2, Dup (Cdt1), and Orc2. Interactions were also detected between Mcm10 and itself, Cdc45, and Hp1. RNAi depletion of Orc2 and Mcm10 in KC cells results in loss of DNA content. Furthermore, depletion of Mcm10, Cdc45, Mcm2, Mcm5, and Orc2, respectively, results in aberrant chromosome condensation. The condensation defects observed resemble previously published reports for Orc2, Orc5, and Mcm4 mutants. Our results strengthen and extend the argument that the processes of chromatin condensation and DNA replication are linked.

引用

页码：2206 / 2215

页数：10

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