A sensitive bioassay for chicken interleukin-18 based on the inducible release of preformed interferon-γ

被引：16
|
作者：
Puehler, F
Göbel, T
Breyer, U
Ohnemus, A
Staeheli, P
Kaspers, B
机构：
[1] Univ Munich, Inst Anim Physiol, D-80539 Munich, Germany
[2] Univ Freiburg, Dept Virol, D-79104 Freiburg, Germany
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 2003年 / 274卷 / 1-2期
关键词：
chicken; interleukin-18; interferon-gamma; bioassay;
D O I：
10.1016/S0022-1759(02)00515-X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Conventional tests for measuring the biological activity of chicken interleukin (IL)-18 require primary chicken spleen cells. We now describe a sensitive bioassay that is based on interleukin-18 -induced release of interferon (IFN)-gamma by a permanent chicken cell line. In B19-2D8 cells, cytoplasmically stored interferon-gamma is quickly secreted in response to interleukin-18 exposure. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：229 / 232
页数：4
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