Construction of Fe-aminoclay-glucose Oxidase Nanocomposite Catalyst and Its Multi-enzyme Cascade Analysis

Glucose oxidase (GO(x)) and Fe-aminoclay (FeAC) were combined to construct a mimic multi-enzyme system (FeAC-GO(x)) for cascade reaction. FeAC exhibit peroxidase-like activity which can catalyze H2O2 generated by GOx and trigger color reaction. The obtained FeAC-GO(x) was characterized by scanning electron microscope (SEM), X-ray diffraction (XRD), and fourier transform infrared spectroscopy (FTIR). The enzymatic kinetics, catalytic stability and reusability were evaluated. As a result, the immobilization efficiency of GO(x) could reach to 76.4%, and FeAC-GO(x) exhibited excellent cascade catalysis activity. Compared with the free enzyme, FeAC not only possessed a strong tolerance toward temperature and pH, but also presented excellent reusability. The catalytic activity of reused FeAC barely changed after six repetitions. This strategy may lay the foundation to the development of a new glucose biosensor, and provide a novel approach to design multi-enzyme cascade system.