Product Diversity Linked to Substrate Usage in Chain Elongation by Mixed-Culture Fermentation

被引:120
作者
Coma, Marta [1 ,3 ]
Vilchez-Vargas, Ramiro [1 ]
Roume, Hugo [1 ]
Jauregui, Ruy [2 ]
Pieper, Dietmar H. [2 ]
Rabaey, Korneel [1 ]
机构
[1] Univ Ghent, Ctr Microbial Ecol & Technol, Coupure Links 653, B-9000 Ghent, Belgium
[2] Helmholtz Ctr Infect Res, Dept Mol Infect Biol, Microbial Interact & Proc Grp, Inhoffenstr 7, D-38124 Braunschweig, Germany
[3] Univ Bath, CSCT, Bath BA2 7AY, Avon, England
基金
欧洲研究理事会;
关键词
REACTOR MICROBIOMES; CLOSTRIDIUM-KLUYVERI; ANAEROBIC-DIGESTION; CARBOXYLIC-ACIDS; HEXANOIC ACID; DYNAMICS; BUTYRATE; KINETICS; ETHANOL; WASTE;
D O I
10.1021/acs.est.5b06021
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Acetate and ethanol can be converted to caproic acid by microorganisms through reverse beta-oxidation. There is limited insight into the versatility of chain elongation in view of different starting substrates, including even- and odd-carbon carboxylates and alcohols other than ethanol. Thermodynamic analyses show that most elongation pathways are energetically feasible. Through incubations of microbial communities with different substrate-pair combinations, we established that ethanol and propanol were both highly suitable for chain elongation. As an electron acceptor, acetate, propionate, and butyrate readily elongated with ethanol, whereas an adaptation period was necessary for formate. Isobutyrate and longer-chained fatty acids above butyrate were not elongated. The microbial communities converged, and consistent enrichment of Clostridium spp. was observed, independent of the supplied alcohol or carboxylate, with a strain related to Clostridium kluyveri dominating the enrichments. Community analysis also showed phylotypes related to Bacteroidaceae and Microbacteriaceae families in all tests that are capable of converting the base substrates to useful intermediates. These organisms were mainly enriched with methanol or formate. Our overall conclusion is thus that multiple substrates can be used for chain elongation and that this process is carried out by highly similar organisms for direct chain elongation irrespective of the substrate.
引用
收藏
页码:6467 / 6476
页数:10
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