Design of multifunctionalized γ-Fe2O3@SiO2 core-shell nanoparticles for enzymes immobilization

被引:34
作者
Georgelin, Thomas [1 ]
Maurice, Vincent [1 ]
Malezieux, Bernard [1 ]
Siaugue, Jean-Michel [1 ]
Cabuil, Valerie [1 ]
机构
[1] Univ Paris 06, Lab Physicochim Electrolytes Colloides & Sci Anal, UMR 7195, F-75252 Paris 05, France
关键词
Magnetic nanoparticles; Silica core-shell nanoparticles; Beta-glucosidase; Enzymatic activity; Multifunctionalization; Nanomedicine; IRON-OXIDE NANOPARTICLES; MAGNETIC NANOPARTICLES;
D O I
10.1007/s11051-009-9757-0
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
This article deals with the first covalent grafting of an enzyme on twice functionalized gamma-Fe2O3@SiO2 core-shell magnetic nanoparticles. First, amino-PEG functionalized nanoparticles were synthesized in order to comply with non-toxic platforms that would be stable in high concentration and would exhibit chemical groups to allow further coupling with biomolecules. This approach produces a colloidal suspension of covalently grafted enzymes that remains stable for months and mimics the enzyme-substrate interactions in solution. Secondly, nanoparticles synthesis and enzyme coupling process were reported and the catalytic properties of bound enzymes were measured and compared with that of the free one. These new materials appear to be useful tools for enzymatic catalysis research and may be extended to other biomolecules. Furthermore, magnetic properties of these materials open the way to separation, purification, and transport under magnetic field.
引用
收藏
页码:675 / 680
页数:6
相关论文
共 16 条
[1]   MAGNETIC COLLOIDAL PROPERTIES OF IONIC FERROFLUIDS [J].
BACRI, JC ;
PERZYNSKI, R ;
SALIN, D ;
CABUIL, V ;
MASSART, R .
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 1986, 62 (01) :36-46
[2]  
Copeland R. A., 2000, Enzyme: A Practical Introduction to Structure, Mechanism, and Data Analysis, V2nd
[3]   BETA-GLUCOSIDASE - SUBSTRATE, SOLVENT, AND VISCOSITY VARIATION AS PROBES OF THE RATE-LIMITING STEPS [J].
DALE, MP ;
KOPFLER, WP ;
CHAIT, I ;
BYERS, LD .
BIOCHEMISTRY, 1986, 25 (09) :2522-2529
[4]   Magnetic nanoparticles for drug delivery [J].
Dobson, J .
DRUG DEVELOPMENT RESEARCH, 2006, 67 (01) :55-60
[5]  
Fauconnier N, 1996, PROG COLL POL SCI S, V100, P212
[6]   Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications [J].
Gupta, AK ;
Gupta, M .
BIOMATERIALS, 2005, 26 (18) :3995-4021
[7]   Direct binding and characterization of lipase onto magnetic nanoparticles [J].
Huang, SH ;
Liao, MH ;
Chen, DH .
BIOTECHNOLOGY PROGRESS, 2003, 19 (03) :1095-1100
[8]   Magnetic iron oxide nanoparticles: Synthesis, stabilization, vectorization, physicochemical characterizations, and biological applications [J].
Laurent, Sophie ;
Forge, Delphine ;
Port, Marc ;
Roch, Alain ;
Robic, Caroline ;
Elst, Luce Vander ;
Muller, Robert N. .
CHEMICAL REVIEWS, 2008, 108 (06) :2064-2110
[9]   Modifying the surface properties of superparamagnetic iron oxide nanoparticles through a sol-gel approach [J].
Lu, Y ;
Yin, YD ;
Mayers, BT ;
Xia, YN .
NANO LETTERS, 2002, 2 (03) :183-186
[10]   Synthesis and characterization of functionalized core-shell γFe2O3-SiO2 nanoparticles [J].
Maurice, Vincent ;
Georgelin, Thomas ;
Siaugue, Jean-Michel ;
Cabuil, Valerie .
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 2009, 321 (10) :1408-1413