Peripheral blood mononuclear cell-converted induced pluripotent stem cells (iPSCs) from an early onset Alzheimer's patient

被引：14

作者：

Lee, Han-Kyu ^{[1
,2
,3
]}

Morin, Peter ^{[3
]}

Xia, Weiming ^{[3
]}

机构：

[1] Rhode Isl Hosp, Dept Neurol, 593 Eddy St, Providence, RI USA

[2] Brown Univ, Warren Alpert Med Sch, 593 Eddy St, Providence, RI 02912 USA

[3] Edith Nourse Rogers Mem Vet Hosp, Geriatr Res Educ & Clin Ctr, Bedford, MA 01730 USA

来源：

STEM CELL RESEARCH | 2016年 / 16卷 / 02期

关键词：

SENDAI-VIRUS; DIFFERENTIATION; GENERATION; DERIVATION; INDUCTION;

D O I：

10.1016/j.scr.2015.12.050

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Improvement in transduction efficiency makes it possible to convert blood cells into induced pluripotent stem cells (iPSC). In this study, we generated an iPSC line from peripheral blood mononuclear cells (PBMC) donated by a patient who exhibited memory deficit at age 59; outcome of positron emission tomography scan is consistent with a diagnosis of Alzheimer's disease. Integration-free CytoTune-iPS Sendai Reprogramming factors which include Sendai virus particles of the four Yamanaka factors Oct4, Sox2, Klf4, and c-Myc were introduced to PBMC to convert them to iPSCs without retention of virus. Three germ layer differentiation was induced to demonstrate the pluripotency of these iPSCs. Published by Elsevier B.V.

引用

页码：213 / 215

页数：3

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