Prevalence, antibiotic resistance and virulence of Enterococcus spp. in Egyptian fresh raw milk cheese

被引:68
作者
Hammad, Ahmed M. [1 ]
Hassan, Harndy A. [2 ,3 ]
Shimamoto, Tadashi [4 ]
机构
[1] Univ Sadat City, Lab Food Hyg & Control, Fac Vet Med, Sadat City, Egypt
[2] Univ Sadat City, Genet Engn & Biotechnol Res Inst, Dept Environm Biotechnol, Sadat City, Egypt
[3] Shaqra Univ, Fac Sci & Humanity Studies Al Quwayiyah, Dept Biol Sci, Al Quwayiyah 11971, Saudi Arabia
[4] Hiroshima Univ, Grad Sch Biosphere Sci, Lab Food Microbiol & Hyg, Higashihiroshima, Hiroshima 7398528, Japan
关键词
Antibiotic resistance; Virulence; Enterococci; Karish cheese; MULTIPLEX PCR; ANTIMICROBIAL RESISTANCE; AMINOGLYCOSIDE RESISTANCE; GENES; DETERMINANTS; FOOD; PERSISTENCE; FAECALIS; LEVEL; CAIRO;
D O I
10.1016/j.foodcont.2014.10.020
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Little is known about the influx of multidrug and/or virulent enterococcal strains from fresh raw milk cheese to the human digestive tract. In this study, 100 samples of popular Egyptian raw milk cheese, karish cheese, were analyzed to determine the incidence of Enterococcus spp., especially Enterococcus faecalis and Enterococcus faecium. We recovered 120 enterococcal isolates from 90 (90%, 90/100) samples. Thirty-seven strains (30.8%, 37/120), were identified as E. faecium and 13 (10.8%, 13/120) as E. faecalis. Fourteen (11.6%, 14/120) strains carried antibiotic resistance genes. These included tet(M), tet(L), tet(K), erm(B), and aph(3'), which were detected in 5%, 3.3%, 0.83%, 0.83%, and 0.83% of isolates, respectively. Interestingly, resistance to the most recently introduced anti-Gram-positive agent, linezolid, was detected alone and in combination with vancomycin resistance in 6.6% and 2.5% of isolated strains, respectively. Additionally, eight strains of E. faecalis carried one or more virulence genes, including asa1, cylA, esp, gelE, and hyl, while only one E. faecium strain carried gelE. Tn916-like elements detected in 11 (9.3%, 11/120) enterococcal strains. In conclusion, the fresh raw milk cheese, karish cheese, is a potential reservoir of antibiotic resistant and virulent enterococci that may constitute a public health hazard. To the best of our knowledge, this study provides the first detailed analysis about the ecology of antibiotic resistance and virulence in a variety of enterococci isolated from fresh raw milk cheese in Africa that promoting the necessary criteria for the phenotypic and molecular analysis of enterococci in this type of cheese to ensure their safety. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:815 / 820
页数:6
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