Comparison of antisense oligonucleotides and siRNAs in cell culture and in vivo

被引：356
作者：
Bertrand, JR
Pottier, M
Vekris, A
Opolon, P
Maksimenko, A
Malvy, C
机构：
[1] Inst Gustave Roussy, CNRS, UMR 8121, Lab Biochim Enzymol, FR-94800 Villejuif, France
[2] DiGEM, R&D 229, F-33076 Bordeaux, France
[3] BioAlliance Pharma SA, F-75015 Paris, France
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 296卷 / 04期
关键词：
antisense oligonucleotide; siRNA; interference; GFP; tumor; HeLa; mice;
D O I：
10.1016/S0006-291X(02)02013-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Efficiencies of a nuclease resistant antisense oligonucleotide and of siRNA both being targeted against the green fluorescent protein stably expressed in HeLa cells are compared in cell cultures and in xenografted mice. Using Cytofectin GSV to deliver both inhibitors, the siRNAs appear to be quantitatively more efficient and its effect is lasting for a longer time in cell culture. In mice, we observed an activity of siRNAs but not of antisense oligonucleotides. The absence of efficiency of antisense oligonucleotides is probably due to their lower resistance to nuclease degradation. (C) 2002 Elsevier Science (USA). All rights reserved.
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收藏
页码：1000 / 1004
页数：5
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