Enhancing freezing tolerance of Brassica napus L. by overexpression of a stearoyl-acyl carrier protein desaturase gene (SAD) from Sapium sebiferum (L.) Roxb.

被引：25

作者：

Peng, Dan ^{[1
,4
]}

Zhou, Bo ^{[1
,2
,3
,4
]}

Jiang, Yueqiao ^{[1
]}

Tan, XiaoFeng ^{[2
,3
]}

Yuan, DeYi ^{[2
,3
]}

Zhang, Lin ^{[2
,3
]}

机构：

[1] Cent South Univ Forestry & Technol, Coll Biosci & Biotechnol, Changsha 410018, Hunan, Peoples R China

[2] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410018, Hunan, Peoples R China

[3] Cent South Univ Forestry & Technol, Collaborat Innovat Cent Cultivat & Utilizat Nonwo, Changsha 410018, Hunan, Peoples R China

[4] Forestry Biotechnol Hunan Key Labs, Changsha 410018, Hunan, Peoples R China

来源：

PLANT SCIENCE | 2018年 / 272卷

基金：

中国国家自然科学基金;

关键词：

Freezing tolerance; Sapium sebiferum (L.) Roxb; Stearoyl-acyl carrier protein desaturase; Unsaturated fatty acid; LOW-TEMPERATURE; COLD-ACCLIMATION; MEMBRANE-LIPIDS; SEED OIL; ARABIDOPSIS; ACID; EXPRESSION; STRESS; PLANT; SENSITIVITY;

D O I：

10.1016/j.plantsci.2018.03.028

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sapium sebiferum (L.) Roxb. is an important woody oil tree and traditional herbal medicine in China. Stearoylacyl carrier protein desaturase (SAD) is a dehydrogenase enzyme that plays a key role in the transformation of saturated fatty acids into unsaturated fatty acids in oil; these fatty acids greatly influence the freezing tolerance of plants. However, it remains unclear whether freezing tolerance can be regulated by the expression level of SsSAD in S. sebiferum L. Our research indicated that SsSAD expression in S. sebiferum L. increased under freezing stress. To further confirm this result, we constructed a pEGAD-SsSAD vector and transformed it into B. napus L. W/O by Agrobacterium tumefaciens-mediated transformation. Transgenic plants that overexpressed the SsSAD gene exhibited significantly higher linoleic (18:2) and linolenic acid (18:3) content and advanced freezing tolerance. These results suggest that SsSAD overexpression in B. napus L. can increase the content of polyunsaturated fatty acids (PUFAs) such as linoleic (18:2) and linolenic acid (18:3), which are likely pivotal in improving freezing tolerance in B. napus L. plants. Thus, SsSAD overexpression could be useful in the production of freeze-tolerant varieties of B. napus L.

引用

页码：32 / 41

页数：10

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