False-positive rifampicin resistance on Xpert® MTB/RIF caused by a silent mutation in the rpoB gene

被引：48

作者：

Mathys, V. ^{[1
]}

van de Vyvere, M. ^{[2
]}

de Droogh, E. ^{[3
]}

Soetaert, K. ^{[1
]}

Groenen, G. ^{[4
]}

机构：

[1] Sci Inst Publ Hlth, Natl Reference Ctr TB & Mycobacteria Communicable, Brussels, Belgium

[2] Ziekenhuis Netwerk Antwerpen ZNA Stuivenberg, Dept Microbiol, Antwerp, Belgium

[3] ZNA Middelheim, Dept Pneumol, Antwerp, Belgium

[4] Belgian Lung & TB Assoc, Brussels, Belgium

来源：

INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE | 2014年 / 18卷 / 10期

关键词：

tuberculosis; molecular test; gene sequencing; MYCOBACTERIUM-TUBERCULOSIS;

D O I：

10.5588/ijtld.14.0297

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The Xpert (R) MTB/RIF assay detects the presence of Mycobacterium tuberculosis and its resistance to rifampicin (RMP) directly in sputum samples. Discrepant results were observed in a case of smear-positive pulmonary tuberculosis that was Xpert-resistant but phenotypically susceptible to RMP. Complementary investigations (repeat Xpert, Genotype (R) MTBDRplus assay and sequencing of the rpoB gene) revealed the presence of a silent mutation in the rpoB gene, leading to the conclusion of a false-positive Xpert result. As misinterpretation of Xpert results may lead to inappropriate treatment, the presence of rpoB mutations should be confirmed by sequencing the rpoB gene.

引用

页码：1255 / 1257

页数：3

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