Discovery and characterization of a novel CCND1/MRCK gene fusion in mantle cell lymphoma

被引：8

作者：

Masamha, Chioniso Patience ^{[1
]}

Albrecht, Todd R. ^{[2
]}

Wagner, Eric J. ^{[2
]}

机构：

[1] Butler Univ, Coll Pharm & Hlth Sci, Dept Pharmaceut Sci, 4600 Sunset Ave, Indianapolis, IN 46208 USA

[2] Univ Texas Med Branch, Dept Biochem & Mol Biol, 301 Univ Blvd, Galveston, TX 77555 USA

来源：

JOURNAL OF HEMATOLOGY & ONCOLOGY | 2016年 / 9卷

基金：

美国国家卫生研究院;

关键词：

Mantle cell lymphoma; Gene fusion; Cyclin D1; miRNA; Alternative polyadenylation; 3 ' UTR; MESSENGER-RNA;

D O I：

10.1186/s13045-016-0260-7

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The t(11; 14) translocation resulting in constitutive cyclin D1 expression is an early event in mantle cell lymphoma (MCL) transformation. Patients with a highly proliferative phenotype produce cyclin D1 transcripts with truncated 3'UTRs that evade miRNA regulation. Here, we report the recurrence of a novel gene fusion in MCL cell lines and MCL patient isolates that consists of the full protein coding region of cyclin D1 (CCND1) and a 3'UTR consisting of sequences from both the CCND1 3'UTR and myotonic dystrophy kinase-related Cdc42-binding kinase's (MRCK) intron one. The resulting CCND1/MRCK mRNA is resistant to CCND1-targeted miRNA regulation, and targeting the MRCK region of the chimeric 3'UTR with siRNA results in decreased CCND1 levels.

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页数：5

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