Glucose biosensor based on nano-SiO2 and "unprotected" Pt nanoclusters

被引：51
作者：
Yang, Haipeng
Zhu, Yongfa [1 ]
机构：
[1] Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China
[2] Shenzhen Univ, Coll Mat Sci & Engn, Shenzhen Key Lab Special Funct Mat, Shenzhen 518060, Peoples R China
来源：
BIOSENSORS & BIOELECTRONICS | 2007年 / 22卷 / 12期
基金：
中国国家自然科学基金;
关键词：
Unprotected Pt nanoclusters; glucose oxidase; glucose biosensor; nanoscale SiO2;
D O I：
10.1016/j.bios.2006.12.019
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
The "unprotected" Pt nanoclusters (average size 2 nm) mixed with the nanoscale SiO2 particles (average size 13 nm) were used as a glucose oxidase immobilization carrier to fabricate the amperometric glucose biosensor. The bioactivity of glucose oxidase (GOx) immobilized on the composite was maintained and the as-prepared biosensor demonstrated high sensitivity (3.85 mu A mM(-1)) and good stability in glucose solution. The Pt-SiO2 biosensor showed a detection limit of 1.5 mu M with a linear range from 0.27 to 4.08 mM. In addition, the biosensor can be operated under wide pH range (pH 4.9-7.5) without great changes in its sensitivity. Cyclic voltammetry measurements showed a n-dxed controlled electrode reaction. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：2989 / 2993
页数：5
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