Identification of candidate genes for an early-maturing soybean mutant by genome resequencing analysis

被引:23
作者
Lee, Kyung Jun [1 ,2 ]
Kim, Dong Sub [1 ]
Kim, Jin-Baek [1 ]
Jo, Sung-Hwan [4 ]
Kang, Si-Yong [1 ]
Choi, Hong-Il [1 ]
Ha, Bo-Keun [3 ]
机构
[1] Korea Atom Energy Res Inst, Radiat Breeding Res Team, Adv Radiat Technol Inst, Jeongeup 580185, South Korea
[2] RDA, NAAS, Natl Agrobiodivers Ctr, Jeonju 560500, South Korea
[3] Chonnam Natl Univ, Div Plant Biotechnol, Coll Agr & Life Sci, Gwangju 500757, South Korea
[4] Seeders Inc, Daejeon 305509, South Korea
基金
新加坡国家研究基金会;
关键词
Early flowering; Gamma-ray; Mutation; Resequencing; Soybean; MOLECULAR CHARACTERIZATION; POSITIONAL CLONING; GAMMA-IRRADIATION; CIRCADIAN-RHYTHMS; DNA; ARABIDOPSIS; CLOCK; TOC1; EXPRESSION; SELECTION;
D O I
10.1007/s00438-016-1183-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Flowering is indicative of the transition from vegetative to reproductive phase, a critical event in the life cycle of plants. In this study, we performed whole genome resequencing by Illumina HiSeq to identify changes in flowering genes using an early-flowering phenotype of soybean mutant line Josaengserori (JS) derived from Korean landrace, Seoritae (SR), and we obtained mapped reads of 131,769,690 and 167,669,640 bp in JS and SR, respectively. From the whole genome sequencing results between JS and SR, we identified 332,821 polymorphic SNPs and 65,178 indels, respectively. Among these, 30 flowering genes were in SNPs and 25 were in indels. Among 30 flowering genes detected in SNPs, Glyma02g33040, Glyma06g22650, Glyma10g36600, Glyma13g01290, Glyma14g10530, Glyma16g01980, Glyma17g11040, Glyma18g53690, and Glyma20g29300 were non-synonymous substitutions between JS and SR. Changes in Glyma10g36600 (GI), Glya02g33040 (AGL18), Glyma17g11040 (TOC1), and Glyma14g10530 (ELF3) in JS affected the expression of GmFT2a and resulted in early flowering. These results provide insight into the regulatory pathways of flowering in soybean mutants and help to improve our knowledge of soybean mutation breeding.
引用
收藏
页码:1561 / 1571
页数:11
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