Effect of introns and AT-rich sequences on expression of the bacterial hygromycin B resistance gene in the basidiomycete Schizophyllum commune

被引：49
作者：
Scholtmeijer, K
Wösten, HAB
Springer, J
Wessels, JGH
机构：
[1] Univ Groningen, Biomol Sci & Biotechnol Inst, Plant Mol Biol Lab, NL-9751 NN Haren, Netherlands
[2] DLO, ATO, Dept Ind Microbiol, NL-6700 AA Wageningen, Netherlands
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2001年 / 67卷 / 01期
关键词：
D O I：
10.1128/AEM.67.1.481-483.2001
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Previously, it was shown that introns are required for efficient mRNA accumulation in Schizophyllum commune and that the presence of AT-rich sequences in the coding region of genes can result in truncation of transcripts in this homobasidiomycete. Here we show that intron-dependent mRNA accumulation and truncation of transcripts are two independent events that both affect expression of the bacterial hygromycin B resistance gene in S. commune.
引用
收藏
页码：481 / 483
页数：3
相关论文
共 14 条
[1]   A comparison of DNA methylation levels in selected isolates of higher fungi [J].
Binz, T ;
D'Mello, N ;
Horgen, PA .
MYCOLOGIA, 1998, 90 (05) :785-790
[2]   Agrobacterium tumefaciens-mediated transformation of filamentous fungi [J].
de Groot, MJA ;
Bundock, P ;
Hooykaas, PJJ ;
Beijersbergen, AGM .
NATURE BIOTECHNOLOGY, 1998, 16 (09) :839-842
[3]   CHARACTERIZATION OF THE GENOME OF THE BASIDIOMYCETE SCHIZOPHYLLUM-COMMUNE [J].
DONS, JJM ;
DEVRIES, OMH ;
WESSELS, JGH .
BIOCHIMICA ET BIOPHYSICA ACTA, 1979, 563 (01) :100-112
[4]   Analysis of heterologous protein production in defined recombinant Aspergillus awamori strains [J].
Gouka, RJ ;
Punt, PJ ;
Hessing, JGM ;
vandenHondel, CAMJJ .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (06) :1951-1957
[5]   Introns are necessary for mRNA accumulation in Schizophyllum commune [J].
Lugones, LG ;
Scholtmeijer, K ;
Klootwijk, R ;
Wessels, JGH .
MOLECULAR MICROBIOLOGY, 1999, 32 (04) :681-689
[6]   INTRODUCTION OF HYGROMYCIN-B RESISTANCE INTO SCHIZOPHYLLUM-COMMUNE - PREFERENTIAL METHYLATION OF DONOR DNA [J].
MOOIBROEK, H ;
KUIPERS, AGJ ;
SIETSMA, JH ;
PUNT, PJ ;
WESSELS, JGH .
MOLECULAR & GENERAL GENETICS, 1990, 222 (01) :41-48
[7]   UPSTREAM INTRONS INFLUENCE THE EFFICIENCY OF FINAL INTRON REMOVAL AND RNA 3'-END FORMATION [J].
NESIC, D ;
MAQUAT, LE .
GENES & DEVELOPMENT, 1994, 8 (03) :363-375
[8]   TRANSFORMATION OF ASPERGILLUS BASED ON THE HYGROMYCIN-B RESISTANCE MARKER FROM ESCHERICHIA-COLI [J].
PUNT, PJ ;
OLIVER, RP ;
DINGEMANSE, MA ;
POUWELS, PH ;
VANDENHONDEL, CAMJJ .
GENE, 1987, 56 (01) :117-124
[9]   Expression of heterologous genes in Schizophyllum commune is often hampered by the formation of truncated transcripts [J].
Schuren, FHJ ;
Wessels, JGH .
CURRENT GENETICS, 1998, 33 (02) :151-156
[10]   HIGHLY-EFFICIENT TRANSFORMATION OF THE HOMOBASIDIOMYCETE SCHIZOPHYLLUM-COMMUNE TO PHLEOMYCIN RESISTANCE [J].
SCHUREN, FHJ ;
WESSELS, JGH .
CURRENT GENETICS, 1994, 26 (02) :179-183
12