Determination of Free Fatty Acids from Soil by LC-MS with Fluorescence Detection and Atmospheric Pressure Chemical Ionization Identification

被引：7

作者：

Chen, Xiangming ^{[1
]}

Dong, Xiuli ^{[1
]}

You, Jinmao ^{[2
]}

机构：

[1] Binzhou Med Univ, Yantai 264003, Shandong, Peoples R China

[2] Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Shandong, Peoples R China

来源：

CHROMATOGRAPHIA | 2010年 / 72卷 / 1-2期

关键词：

Column liquid chromatography-mass spectrometry; Fluorescence detection; Pre-column derivatization; Fatty acids; PERFORMANCE LIQUID-CHROMATOGRAPHY; CARBOXYLIC-ACIDS; DERIVATIZATION REAGENT; LABELING REAGENT; GAS-CHROMATOGRAPHY; 9-ANTHRYLDIAZOMETHANE; DERIVATIVES; SEPARATION;

D O I：

10.1365/s10337-010-1610-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and highly sensitive method for the determination of fatty acids using liquid chromatography with fluorescence detection is described. Fatty acids were derivatized by a new novel pre-column derivatization reagent 1,2-benzo-3,4-dihydrocarbazole-9-ethanol before injection. Derivatives were sufficiently stable for efficient analysis by LC without pre-treatment. On a reversed-phase XDB-C(8) column, 19 kinds of fatty acids (C(1)-C(19)) derivatives were separated in conjunction with gradient elution and good baseline resolution. The precise determination of fatty acids from soil sample was achieved by post-column online mass spectrometric identification with atmospheric pressure chemical ionization source in positive mode. The established method exhibited good selectivity and excellent repeatability. The RSD (n = 6) for each fatty acid derivative were < 3%, detection limits (at signal-to-noise of 3:1) were 11.79-33.04 fmol.

引用

页码：171 / 175

页数：5

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