High expression of Bcl-x protein in the developing human cerebellar cortex

被引：0
作者：
Sohma, O
Mizuguchi, M
Takashima, S
Yamada, M
Ikeda, K
Ohta, S
机构：
[1] NCNP,NATL INST NEUROSCI,DEPT MENTAL RETARDAT & BIRTH DEFECT RES,KODAIRA,TOKYO 187,JAPAN
[2] TOKYO INST PSYCHIAT SETAGAYA,DEPT ULTRASTRUCT & HISTOCHEM,TOKYO,JAPAN
[3] NIIGATA UNIV,BRAIN RES INST,DEPT PATHOL,NIIGATA,JAPAN
[4] NIPPON MED COLL,INST GERONTOL,DIV BIOCHEM,KAWASAKI,KANAGAWA,JAPAN
来源：
JOURNAL OF NEUROSCIENCE RESEARCH | 1996年 / 43卷 / 02期
关键词：
apoptosis; central nervous system; development; immunochemistry; immunohistochemistry;
D O I：
10.1002/(SICI)1097-4547(19960115)43:2<175::AID-JNR5>3.0.CO;2-D
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The gene bcl-x, which is related to a bcl-2, regulates programmed cell death, bcl-x may function in the development of the nervous system, We raised a polyclonal antibody against human Bcl-x protein, and investigated its distribution in the developing human cerebellum, Western blotting revealed that Bcl-x expression in the cerebellum is higher in the fetal, than in the postnatal period. Immunohistochemical studies of fetal brains localized intense Bcl-x immunoreactivity in the granule cell processes at 13-22 gestational weeks and in the Purkinje cell bodies at 24-38 weeks. Tbe immunoreactivity decreased after birth, but was retained in the Purkinje cells at a low level until adulthood, These results suggested that Bcl-x expression in the cerebellum is developmentally regulated and involved specifically in the development of neuronal subpopulations. (C) 1996 Wiley-Liss, Inc.
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页码：175 / 182
页数：8
相关论文
共 28 条
[1]   BCL-2 GENE IS HIGHLY EXPRESSED DURING NEUROGENESIS IN THE CENTRAL-NERVOUS-SYSTEM [J].
ABEDOHMAE, S ;
HARADA, N ;
YAMADA, K ;
TANAKA, R .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 191 (03) :915-921
[2]   THE PROTOONCOGENE BCL-2 CAN SELECTIVELY RESCUE NEUROTROPHIC FACTOR-DEPENDENT NEURONS FROM APOPTOSIS [J].
ALLSOPP, TE ;
WYATT, S ;
PATERSON, HF ;
DAVIES, AM .
CELL, 1993, 73 (02) :295-307
[3]   BCL-X, A BCL-2-RELATED GENE THAT FUNCTIONS AS A DOMINANT REGULATOR OF APOPTOTIC CELL-DEATH [J].
BOISE, LH ;
GONZALEZGARCIA, M ;
POSTEMA, CE ;
DING, LY ;
LINDSTEN, T ;
TURKA, LA ;
MAO, XH ;
NUNEZ, G ;
THOMPSON, CB .
CELL, 1993, 74 (04) :597-608
[4]   BCL-2 MESSENGER-RNA IS LOCALIZED IN NEURONS OF THE DEVELOPING AND ADULT-RAT BRAIN [J].
CASTREN, E ;
OHGA, Y ;
BERZAGHI, MP ;
TZIMAGIORGIS, G ;
THOENEN, H ;
LINDHOLM, D .
NEUROSCIENCE, 1994, 61 (01) :165-177
[5]   REGRESSIVE EVENTS IN NEUROGENESIS [J].
COWAN, WM ;
FAWCETT, JW ;
OLEARY, DDM ;
STANFIELD, BB .
SCIENCE, 1984, 225 (4668) :1258-1265
[6]   DISTRIBUTION OF MICROTUBULE-ASSOCIATED PROTEIN 2 IN THE NERVOUS-SYSTEM OF THE RAT STUDIED BY IMMUNOFLUORESCENCE [J].
DECAMILLI, P ;
MILLER, PE ;
NAVONE, F ;
THEURKAUF, WE ;
VALLEE, RB .
NEUROSCIENCE, 1984, 11 (04) :819-846
[7]   NEONATAL MOTONEURONS OVEREXPRESSING THE BCL-2 PROTOONCOGENE IN TRANSGENIC MICE ARE PROTECTED FROM AXOTOMY-INDUCED CELL-DEATH [J].
DUBOISDAUPHIN, M ;
FRANKOWSKI, H ;
TSUJIMOTO, Y ;
HUARTE, J ;
MARTINOU, JC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (08) :3309-3313
[8]  
Ellis H.M., 1991, ANN REV CELL BIOL, V7, P663, DOI DOI 10.1146/ANNUREV.CB.07.110191.003311
[9]   INCREASED EXPRESSION OF BCL-2 IMMUNOREACTIVITY IN THE DEVELOPING CEREBRAL-CORTEX OF THE RAT [J].
FERRER, I ;
TORTOSA, A ;
CONDOM, E ;
BLANCO, R ;
MACAYA, A ;
PLANAS, A .
NEUROSCIENCE LETTERS, 1994, 179 (1-2) :13-16
[10]   PREVENTION OF PROGRAMMED CELL-DEATH OF SYMPATHETIC NEURONS BY THE BCL-2 PROTOONCOGENE [J].
GARCIA, I ;
MARTINOU, I ;
TSUJIMOTO, Y ;
MARTINOU, JC .
SCIENCE, 1992, 258 (5080) :302-304
123