Assessment of immunization response to canine distemper virus vaccination in puppies using a clinic-based enzyme-linked immunosorbant assay

被引:20
|
作者
Waner, T
Naveh, A
Ben Meir, NS
Babichev, Z
Carmichael, LE
机构
[1] Israel Inst Biol Res, IL-70450 Ness Ziona, Israel
[2] Biol Labs, IL-19240 Kibbutz Galed, MP Megiddo, Israel
[3] Cornell Univ, New York State Coll Vet Med, James A Baker Inst Anim Hlth, Ithaca, NY 14853 USA
关键词
canine distemper virus; ELISA; vaccination failure; maternal antibody;
D O I
10.1016/S1090-0233(98)80013-0
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
To study the response to vaccination, an enzyme-linked immunosorbant assay (ELISA) immunoblot method was developed and tested to assay canine distemper virus (CDV) IgG antibody in puppies and compared to a standard virus neutralization (VN) test (r(2)=0.748). Ten litters of four puppies each were used in a vaccination study. Seventy-six percent of vaccinated puppies immunized with a modified live vaccine were successfully protected against CDV at 6 weeks of age. One puppy remained seronegative after vaccination at 6 and 9 weeks of age. This is the first report of vaccination responses of puppies to CDV using an in-clinic test kit based on solid-phase immunoassay technology.
引用
收藏
页码:171 / 175
页数:5
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