A multiplex PCR to differentiate the two sibling species of mosquitoes Ochlerotatus detritus and Oc. coluzzii and evidence for further genetic heterogeneity within the Detritus complex

被引：11

作者：

Brengues, C. ^{[1
]}

Ferre, J. B. ^{[2
]}

Le Goff, G. ^{[1
]}

Lami, P. ^{[1
,3
]}

Pratlong, F. ^{[1
,3
]}

Pasteur, N. ^{[4
]}

Lagneau, C. ^{[2
]}

Simard, F. ^{[1
]}

Robert, V. ^{[1
]}

机构：

[1] Univ Montpellier I, Univ Montpellier 2, MIVEGEC, UMR IRD 224,CNRS 5290, Montpellier, France

[2] EID Mediterranee, Montpellier, France

[3] CHRU Montpellier, Lab Parasitol Mycol, Ctr Natl Reference Leishmanioses, Montpellier, France

[4] Univ Montpellier 2, CNRS, ISEM UMR 5554, F-34095 Montpellier 5, France

来源：

INFECTION GENETICS AND EVOLUTION | 2014年 / 28卷

关键词：

Mosquito; Aedini; Species determination; Multiplex PCR; AEDES-DETRITUS; DIPTERA; IDENTIFICATION; CULICIDAE; VECTORS; AEDINI; DELTA;

D O I：

10.1016/j.meegid.2014.07.010

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Internal transcribed spacer regions of ribosomal DNA were sequenced, and species-specific primers were designed to simplify the identification of two morphologically similar species of the Detritus complex, Ochlerotatus detritus and Ochlerotatus coluzzii. Each newly designed primer was able to amplify a species-specific fragment with a different size. Samples from France and Germany were successfully tested. This new tool prompts for bio-ecological studies to refine basic knowledge on the bionomics of this species complex, towards a better control and prevention of ensuing nuisances. Moreover, ITS2 sequencing revealed the existence of (1) two distinct haplotypes of Oc. detritus that are sympatric and widely distributed along the French Atlantic and Mediterranean littorals and (2) a specific haplotype in mosquitoes sampled from Tunisia, raising the question of the taxonomic status of this North-African population. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：676 / 680

页数：5

共 24 条

[1]

Agoulon A, 1999, ANN SOC ENTOMOL FR, V35, P263

[2]

Brunhes J., 2000, Bulletin de la Societe Entomologique de France, V105, P195

[3] IDENTIFICATION OF 2 SIBLING SPECIES A AND B OF AEDES-DETRITUS COMPLEX ON ATLANTIC COASTLINE IN FRANCE [J].

BRUTUS, L ;

RIANDEY, MF ;

GUILLOTEAU, J ;

MONTENY, N ;

SANNIER, C ;

MARJOLET, M .

PARASITE, 1994, 1 (02) :167-170

[4] Species identification within the Anopheles funestus group of malaria vectors in Cameroon and evidence for a new species [J].

Cohuet, A ;

Simard, F ;

Toto, JC ;

Kengne, P ;

Coetzee, M ;

Fontenille, D .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2003, 69 (02) :200-205

[5] A review of the use of ribosomal DNA (rDNA) to differentiate among cryptic Anopheles species [J].

Collins, FH ;

Paskewitz, SM .

INSECT MOLECULAR BIOLOGY, 1996, 5 (01) :1-9

[6] The invaders: Phylogeography of dengue and chikungunya viruses Aedes vectors, on the South West islands of the Indian Ocean [J].

Delatte, H. ;

Bagny, L. ;

Brengue, C. ;

Bouetard, A. ;

Paupy, C. ;

Fontenille, D. .

INFECTION GENETICS AND EVOLUTION, 2011, 11 (07) :1769-1781

[7] CLUSTAL - A PACKAGE FOR PERFORMING MULTIPLE SEQUENCE ALIGNMENT ON A MICROCOMPUTER [J].

HIGGINS, DG ;

SHARP, PM .

GENE, 1988, 73 (01) :237-244

[8] Genetic Structure of the Tiger Mosquito, Aedes albopictus, in Cameroon (Central Africa) [J].

Kamgang, Basile ;

Brengues, Cecile ;

Fontenille, Didier ;

Njiokou, Flobert ;

Simard, Frederic ;

Paupy, Christophe .

PLOS ONE, 2011, 6 (05)

[9] Molecular identification of the Anopheles nili group of African malaria vectors [J].

Kengne, P ;

Awono-Ambene, P ;

Nkondjio, CA ;

Simard, F ;

Fontenille, D .

MEDICAL AND VETERINARY ENTOMOLOGY, 2003, 17 (01) :67-74

[10] Molecular Genetic Investigation of Morphological Species of Members of the Neomelaniconion Subgenus (Diptera: Culicidae: Aedini) From Madagascar Using Ribosomal Internal Transcribed Spacer 2 [J].

Kengne, Pierre ;

Le Goff, Gilbert ;

Fontenille, Didier .

JOURNAL OF MEDICAL ENTOMOLOGY, 2009, 46 (02) :403-407

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