Nonlinear endomicroscopy using a double-clad fiber coupler

被引:21
作者
Bao, Hongchun [1 ]
Ryu, Seon Young [2 ]
Lee, Byeong Ha [3 ]
Tao, Wei [1 ]
Gu, Min [1 ]
机构
[1] Swinburne Univ Technol, Ctr Microphoton, Fac Engn & Ind Sci, Hawthorn, Vic 3122, Australia
[2] Korea Basic Sci Inst, Div Instrument Dev, Taejon 305333, South Korea
[3] Gwangju Inst Sci & Technol, Dept Informat & Commun, Kwangju 500712, South Korea
基金
澳大利亚研究理事会;
关键词
PHOTONIC CRYSTAL FIBER; FLUORESCENCE SPECTROSCOPY; MU-M; MICROSCOPY; ENDOSCOPY; PROBE; SYSTEM; HEAD;
D O I
10.1364/OL.35.000995
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
A double-clad fiber coupler is developed to be used in two-photon-excited fluorescence endomicroscopy to replace a dichroic mirror and separate the fluorescence signal from the excitation laser beam. With the double-clad fiber coupler, the endomicroscope becomes more compact, easier to be aligned, and more stable in alignment. The double-clad fiber coupler can transmit 62% of the excitation laser beam through the core. The fluorescence collection efficiency of the double-clad fiber coupler is 34%, which is, to the best of our knowledge, the highest fluorescence collection efficiency achieved by couplers used in two-photon-excited fluorescence endomicroscopes. As a result, the contrast of endomicroscopy imaging is enhanced. (C) 2010 Optical Society of America
引用
收藏
页码:995 / 997
页数:3
相关论文
共 22 条
[1]   Fast handheld two-photon fluorescence microendoscope with a 475 μm X 475 μm field of view for in vivo imaging [J].
Bao, Hongchun ;
Allen, John ;
Pattie, Robert ;
Vance, Rod ;
Gu, Min .
OPTICS LETTERS, 2008, 33 (12) :1333-1335
[2]   A 0.4-mm-diameter probe for nonlinear optical imaging [J].
Bao, Hongchun ;
Gu, Min .
OPTICS EXPRESS, 2009, 17 (12) :10098-10104
[3]   Reduction of self-phase modulation in double-clad photonic crystal fiber for nonlinear optical endoscopy [J].
Bao, Hongchun ;
Gu, Min .
OPTICS LETTERS, 2009, 34 (02) :148-150
[4]   Two-photon fluorescence endoscopy with a micro-optic scanning head [J].
Bird, D ;
Gu, M .
OPTICS LETTERS, 2003, 28 (17) :1552-1554
[5]   Compact two-photon fluorescence microscope based on a single-mode fiber coupler [J].
Bird, D ;
Gu, M .
OPTICS LETTERS, 2002, 27 (12) :1031-1033
[6]   Fiber-optic fluorescence imaging [J].
Flusberg, BA ;
Cocker, ED ;
Piyawattanametha, W ;
Jung, JC ;
Cheung, ELM ;
Schnitzer, MJ .
NATURE METHODS, 2005, 2 (12) :941-950
[7]   Use of a single-mode fiber coupler for second-harmonic-generation microscopy [J].
Fu, L ;
Gan, XS ;
Gu, M .
OPTICS LETTERS, 2005, 30 (04) :385-387
[8]   Polarization anisotropy in fiber-optic second harmonic generation microscopy [J].
Fu, Ling ;
Gu, Min .
OPTICS EXPRESS, 2008, 16 (07) :5000-5006
[9]   Double-clad photonic crystal fiber coupler for compact nonlinear optical microscopy imaging [J].
Fu, Ling ;
Gu, Min .
OPTICS LETTERS, 2006, 31 (10) :1471-1473
[10]   In-vivo confocal real-time mini-microscopy in animal models of human inflammatory and neoplastic diseases [J].
Goetz, M. ;
Fottner, C. ;
Schirrmacher, E. ;
Delaney, P. ;
Gregor, S. ;
Schneider, C. ;
Strand, D. ;
Kanzler, S. ;
Memadathil, B. ;
Weyand, E. ;
Holtmann, M. ;
Schirrmacher, R. ;
Weber, M. M. ;
Anlauf, M. ;
Kloeppel, G. ;
Vieth, M. ;
Galle, P. R. ;
Bartenstein, P. ;
Neurath, M. F. ;
Kiesslich, R. .
ENDOSCOPY, 2007, 39 (04) :350-356