Discovery of widespread type I and type V CRISPR-Cas inhibitors

被引：191

作者：

Marino, Nicole D. ^{[1
]}

Zhang, Jenny Y. ^{[1
]}

Borges, Adair L. ^{[1
]}

Sousa, Alexander A. ^{[2
,3
,4
]}

Leon, Lina M. ^{[1
]}

Rauch, Benjamin J. ^{[1
,7
]}

Walton, Russell T. ^{[2
,3
,4
]}

Berry, Joel D. ^{[1
,8
]}

Joung, J. Keith ^{[2
,3
,4
,5
]}

Kleinstiver, Benjamin P. ^{[2
,3
,4
,5
]}

Bondy-Denomy, Joseph ^{[1
,6
]}

机构：

[1] Univ Calif San Francisco, Dept Microbiol & Immunol, San Francisco, CA 94143 USA

[2] Massachusetts Gen Hosp, Mol Pathol Unit, Charlestown, MA 02129 USA

[3] Massachusetts Gen Hosp, Ctr Canc Res, Charlestown, MA 02129 USA

[4] Massachusetts Gen Hosp, Ctr Computat & Integrat Biol, Charlestown, MA 02129 USA

[5] Harvard Med Sch, Dept Pathol, Boston, MA 02115 USA

[6] Univ Calif San Francisco, Quantitat Biosci Inst, San Francisco, CA 94143 USA

[7] Zymergen, Emeryville, CA 94608 USA

[8] Caribou Biosci, Berkeley, CA 94710 USA

来源：

SCIENCE | 2018年 / 362卷 / 6411期

基金：

美国国家卫生研究院;

关键词：

INFECTIOUS BOVINE KERATOCONJUNCTIVITIS; CPF1; MECHANISMS; PROTEINS; SYSTEM; RNA;

D O I：

10.1126/science.aau5174

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Bacterial CRISPR-Cas systems protect their host from bacteriophages and other mobile genetic elements. Mobile elements, in turn, encode various anti-CRISPR (Acr) proteins to inhibit the immune function of CRISPR-Cas. To date, Acr proteins have been discovered for type I (subtypes I-D, I-E, and I-F) and type II (II-A and II-C) but not other CRISPR systems. Here, we report the discovery of 12 acr genes, including inhibitors of type V-A and I-C CRISPR systems. AcrVA1 inhibits a broad spectrum of Cas12a (Cpf1) orthologs-including MbCas12a, Mb3Cas12a, AsCas12a, and LbCas12a-when assayed in human cells. The acr genes reported here provide useful biotechnological tools and mark the discovery of acr loci in many bacteria and phages.

引用

页码：240 / 242

页数：3

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