Infection-Specific Activation of the Medicago truncatula Enod11 Early Nodulin Gene Promoter During Actinorhizal Root Nodulation

被引:29
作者
Svistoonoff, Sergio [1 ]
Sy, Mame-Oureye [1 ,2 ]
Diagne, Nathalie [1 ,3 ]
Barker, David G. [4 ]
Bogusz, Didier [1 ]
Franche, Claudine [1 ]
机构
[1] IRD, Unite Mixte Rech DIAPC, Grp Rhizogenese, F-34394 Montpellier 5, France
[2] Univ Cheikh Anta Diop, Fac Sci & Tech, Dept Biol Vegetale, Lab Campus Biotechnol Vegetales, Dakar, Senegal
[3] Ctr Rech Bel Air, Lab Commun Microbiol IRD ISRA UCAD, Dakar, Senegal
[4] UMR INRA CNRS 441 2594, Lab Plant Microbe Interact, F-31326 Castanet Tolosan, France
关键词
ARBUSCULAR MYCORRHIZAL FUNGI; CASUARINA-GLAUCA; ALLOCASUARINA-VERTICILLATA; RHIZOBIAL INFECTION; MTENOD11; EXPRESSION; CONTAINING CELLS; FRANKIA; SYMBIOSIS; TRANSDUCTION; HAIRS;
D O I
10.1094/MPMI-23-6-0740
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The MtEnod11 gene from Medicago truncatula is widely used as an early infection-related molecular marker for endosymbiotic associations involving both rhizobia and arbuscular mycorrhizal fungi. In this article, heterologous expression of the MtEnod11 promoter has been studied in two actinorhizal trees, Casuarina glauca and Allocasuarina verticillata. Transgenic C. glauca and A. verticillata expressing a ProMtEnod11::beta-glucuronidase (gus) fusion were generated and the activation of the transgene investigated in the context of the symbiotic associations with the N-fixing actinomycete Frankia and both endo- and ectomycorrhizal fungi (Glomus intraradices and Pisolithus albus, respectively). ProMtEnod11::gus expression was observed in root hairs, prenodules, and nodules and could be correlated with the infection of plant cells by Frankia spp. However, no activation of the gus reporter gene was detected prior to infection or in response to either rhizobial Nod factors or the wasp venom peptide MAS-7. Equally, ProMtEnod11::gus expression was not elicited during the symbiotic associations with either ecto- or endomycorrhizal fungi. These observations suggest that, although there is a conservation of gene regulatory pathways between legumes and actinorhizal plants in cells accommodating endosymbiotic N-fixing bacteria, the events preceding bacterial infection or related to mycorrhization appear to be less conserved.
引用
收藏
页码:740 / 747
页数:8
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