YB-1 promotes laryngeal squamous cell carcinoma progression by inducing miR-155 expression via c-Myb

被引：23

作者：

Zhao, Xudong ^{[1
]}

Zhang, Wei ^{[2
]}

Ji, Wenyue ^{[1
]}

机构：

[1] China Med Univ, Shengjing Hosp, Dept Otorhinolaryngol, Shenyang 110004, Liaoning, Peoples R China

[2] China Med Univ, Dept Endocrinol, Shengjing Hosp, Shenyang 110004, Liaoning, Peoples R China

来源：

FUTURE ONCOLOGY | 2018年 / 14卷 / 16期

基金：

中国国家自然科学基金;

关键词：

cervical metastasis; c-Myb; head and neck cancer; invasion; laryngeal cancer; LSCC; miR-155; prognosis; proliferation; YB-1; BINDING-PROTEINS; DIAGNOSIS; REGION;

D O I：

10.2217/fon-2018-0058

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Aim: In this study, we investigated the role of Y-box binding protein-1 (YB- 1), c-Myb and miR-155 in human laryngeal squamous cell carcinoma (LSCC) progression. Materials & methods: Quantitative real-time PCR, western blot, MTT and Transwell were conducted to determine the expression and function of YB-1/ miR-155 pathway. Univariate and multivariate analyses were used to determine the prognostic factors. Results: Expression of YB-1, c-Myb and miR-155 was higher in LSCC tissues. YB-1 promoted proliferation, invasiveness and migration of Hep-2 cells in vitro. Patients with higher YB-1 correlated with advanced T stage, poor differentiation and cervical metastasis. LSCC patients with high YB-1 expression showed poor overall survival. Conclusion: YB-1 promotes LSCC progression by increasing miR-155 levels via c-Myb and acts as a prognostic factor.

引用

页码：1579 / 1589

页数：11

共 25 条

[1] MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].

Bartel, David P. .

CELL, 2007, 131 (04) :11-29

[2] PROTEIN TIGHTLY BOUND TO GLOBIN MESSENGER-RNA [J].

BLOBEL, G .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1972, 47 (01) :88-&

[3] Laryngeal cancer: Diagnosis and preoperative work-up [J].

Chu, Eugene A. ;

Kim, Young J. .

OTOLARYNGOLOGIC CLINICS OF NORTH AMERICA, 2008, 41 (04) :673-+

[4] CHARACTERIZATION OF THE CDNA-ENCODING A PROTEIN-BINDING TO THE MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-II Y-BOX [J].

DIDIER, DK ;

SCHIFFENBAUER, J ;

WOULFE, SL ;

ZACHEIS, M ;

SCHWARTZ, BD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (19) :7322-7326

[5] MiR-424 and miR-155 deregulated expression in cytogenetically normal acute myeloid leukaemia: correlation with NPM1 and FLT3 mutation status [J].

Faraoni, Isabella ;

Laterza, Serena ;

Ardiri, Davide ;

Ciardi, Claudia ;

Fazi, Francesco ;

Lo-Coco, Francesco .

JOURNAL OF HEMATOLOGY & ONCOLOGY, 2012, 5

[6]

Ge WS, 2016, AM J TRANSL RES, V8, P3903

[7] Nuclear YB-1 expression as a negative prognostic marker in nonsmall cell lung cancer [J].

Gessner, C ;

Woischwill, C ;

Schumacher, A ;

Liebers, U ;

Kuhn, H ;

Stiehl, P ;

Jürchott, K ;

Royer, HD ;

Witt, C ;

Wolff, G .

EUROPEAN RESPIRATORY JOURNAL, 2004, 23 (01) :14-19

[8] YB-1 as a cell cycle-regulated transcription factor facilitating cyclin A and cyclin B1 gene expression [J].

Jürchott, K ;

Bergmann, S ;

Stein, U ;

Walther, W ;

Janz, M ;

Manni, I ;

Piaggio, G ;

Fietze, E ;

Dietel, M ;

Royer, HD .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (30) :27988-27996

[9] A ROLE FOR Y-BOX PROTEINS IN CELL-PROLIFERATION [J].

LADOMERY, M ;

SOMMERVILLE, J .

BIOESSAYS, 1995, 17 (01) :9-11

[10] YB-1 alters MT1-MMP trafficking and stimulates MCF-7 breast tumor invasion and metastasis [J].

Lovett, David H. ;

Cheng, Sunfa ;

Cape, Leslie ;

Pollock, Allan S. ;

Mertens, Peter R. .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2010, 398 (03) :482-488

← 1 2 3 →