Covalent-attached self-assembly film based on diazoresin and bovine serum albumin and the cell culture on its surface

A novel multilayer film was fabricated from bovine serum albumin(BSA), as polyanion through the adjusting of pH value (pH=7. 0), and photo-sensitive diazoresin (DR), as polycation (pH=6. 5), in aqueous solution via. electrostatic self-assembly. The results show that,the absorbance at 383 nm characterized of diazonium group of multilayer films increased with the,increase of bilayer number. A good linear relationship between absorbance at 383 nm and the bilayer number indicates that a smooth step-by-step fabrication has taken place on the quartz wafer. Under UV irradiation, following the decomposition of diazonium group between the adjacent interfaces of the multilayers, the ionic bonds of the self-assembly film were converted to covalent bonds and the film becomes very stable toward electrolyte aqueous solutions. Due to the cellular affinity of serum albumin, uterine epithelial cell monolayer was successfully cultured on the surface of self-assembled film of DR/BSA.