Cu-doped In2S3 based DNA nanocluster for ultrasensitive photoelectrochemical detection of VEGF165

被引：15

作者：

Ai, Simin ^{[1
]}

Zeng, Hongmei ^{[1
]}

Chai, Yaqin ^{[1
]}

Yuan, Ruo ^{[1
]}

Liu, Hongyan ^{[1
]}

机构：

[1] Southwest Univ, Key Lab Luminescence Anal & Mol Sensing, Coll Chem & Chem Engn, Minist Educ, Chongqing 400715, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2021年 / 340卷

基金：

中国国家自然科学基金;

关键词：

Cu-doped In2S3; Defective material; DNA nanocluster; VEGF165; detection; Signal-off; Photoelectrochemical biosensors; ENDOTHELIAL GROWTH-FACTOR; AMPLIFICATION; ENHANCEMENT;

D O I：

10.1016/j.snb.2021.129942

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this work, defective Cu-doped In2S3 (Cu: In2S3) was explored as photoactive material to fabricate a novel "signal-off" photoelectrochemical (PEC) sensing platform for ultrasensitive vascular endothelial growth factor 165 (VEGF165) detection. The defective Cu: In2S3 generated approximately ten times higher photocurrent than the pristine In2S3, which was attributed to the enhanced visible light absorption and electron-hole separation caused by the negative-charged defects induced by Cu doping. Moreover, an aptamer bridged nanocluster consisting of Cu: In2S3 material and DNA strand was assembled on the electrode as an efficient PEC signal indicator. The immobilized photoactive Cu: In2S3 could be released by recognition of the target VEGF165 to achieve a "signal-off" biosensor. The proposed PEC sensor performs a desirable detection response in the range of 10 fM to 100 nM VEGF165 with a low detection limit of 3 fM. This strategy not only avoided difficult assembly of diverse signal indicator and quenchers, but also significantly improved the detection sensitivity and linear range of PEC biosensor, offering a new perspective for construction of PEC biosensors for accurate, sensitive, and convenient bioanalysis and disease diagnosis.

引用

页数：7

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