Engineering protein A affinity chromatography

被引：1
|
作者：
Jungbauer, A
Hahn, R
机构：
[1] Univ Nat Resources & Appl Life Sci, A-1190 Vienna, Austria
[2] Austrian Ctr Biopharmaceut Technol, Dept Biotechnol, A-1190 Vienna, Austria
来源：
CURRENT OPINION IN DRUG DISCOVERY & DEVELOPMENT | 2004年 / 7卷 / 02期
关键词：
antibody; immunoglobulin; ligand leakage; purification; scale-up; virus removal;
D O I：
暂无
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Staphylococcal protein A can selectively interact with immunoglobulins. This protein is widely used as a ligand for affinity chromatography to purify therapeutic antibodies on an industrial scale. This type of affinity chromatography constitutes a generic step in processing antibodies. Questions of scale-up, design of chromatographic conditions, clearance of adventitious agents and operational modes, such as continuous operation or purification of antibodies in expanded-bed mode, will be addressed in this review.
引用
收藏
页码：248 / 256
页数：9
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