Carboxyl-functionalized magnetic microparticle carrier for isolation and identification of DNA in dairy products

被引：37
作者：
Horak, Daniel
Rittich, Bohuslav
Spanova, Alena
机构：
[1] Acad Sci Czech Republ, Inst Macromol Chem, CR-16206 Prague 6, Czech Republic
[2] Masaryk Univ, Brno 60200, Czech Republic
来源：
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS | 2007年 / 311卷 / 01期
关键词：
magnetic particles; DNA; magnetite; PCR; dispersion polymerization; bacterial DNA assay; assay; DISPERSION POLYMERIZATION; MICROSPHERES; PARTICLES; POLYMERS;
D O I：
10.1016/j.jmmm.2006.10.1157
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
Magnetite nanoparticles about 14 nm in diameter were obtained by chemical coprecipitation of Fe(II) and Fe(III) salts with aqueous ammonia in the presence of poly(ethylene glycol) (PEG). Magnetic poly(glycidyl methacrylate) (PGMA) microspheres about I Pin in diameter were prepared by dispersion polymerization of GMA in aqueous ethanol in the presence of PEG-coated magnetite nanoparticles. The microspheres were hydrolyzed and carboxyl groups introduced by oxidation with KMnO4. The particles reversibly bound bacterial DNA of Bifidobacterium and Lactobacillus genera in the presence of high concentrations of PEG 6000 and sodium chloride from crude cell lysates of various dairy products (butter milk, cheese, yoghurt, probiotic tablets) or from cell lyophilisates. The presence of Bifidobacterium and Lactobacillus DNA in samples was confirmed by PCR amplification. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：249 / 254
页数：6
相关论文
共 27 条
[1]  
[Anonymous], MOL CLONING
[2]  
DAUTZENBERG H, 1974, FASERFORSCH TEXTILTE, V25, P469
[3]   SOLID-PHASE REVERSIBLE IMMOBILIZATION FOR THE ISOLATION OF PCR PRODUCTS [J].
DEANGELIS, MM ;
WANG, DG ;
HAWKINS, TL .
NUCLEIC ACIDS RESEARCH, 1995, 23 (22) :4742-4743
[4]   The magnetic immuno-polymerase chain reaction assay for the detection of Campylobacter in milk and poultry [J].
Docherty, L ;
Adams, MR ;
Patel, P ;
McFadden, J .
LETTERS IN APPLIED MICROBIOLOGY, 1996, 22 (04) :288-292
[5]   A PCR-based method for identification of lactobacilli at the genus level [J].
Dubernet, S ;
Desmasures, N ;
Guéguen, M .
FEMS MICROBIOLOGY LETTERS, 2002, 214 (02) :271-275
[6]  
*GEN SCAN EUR, GMOIDENT ROUND READ
[7]   DNA STRUCTURAL DYNAMICS - LONGITUDINAL BREATHING AS A POSSIBLE MECHANISM FOR THE B-REVERSIBLE-Z TRANSITION [J].
HARVEY, SC .
NUCLEIC ACIDS RESEARCH, 1983, 11 (14) :4867-4878
[8]   Magnetic poly(glycidyl methacrylate) microspheres prepared by dispersion polymerization in the presence of electrostatically stabilized ferrofluids [J].
Horák, D ;
Benedyk, N .
JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, 2004, 42 (22) :5827-5837
[9]  
Horák D, 2000, J POLYM SCI POL CHEM, V38, P3855
[10]   Presentation of a new magnetic field therapy system for the treatment of human solid tumors with magnetic fluid hyperthermia [J].
Jordan, A ;
Scholz, R ;
Maier-Hauff, K ;
Johannsen, M ;
Wust, P ;
Nadobny, J ;
Schirra, H ;
Schmidt, H ;
Deger, S ;
Loening, S ;
Lanksch, W ;
Felix, R .
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 2001, 225 (1-2) :118-126
123