Structural Elucidation and genetic identification of the O-antigen from a novel serogroup of Escherichia coli strain 2017LL031

被引：1
作者：
Qin, Chunjun [1 ]
Hu, Bin [2 ]
Xu, Yujuan [1 ]
Zhao, Changqing [4 ]
Hao, Wei [2 ]
Wang, Jing [5 ]
Guo, Xi [5 ]
Li, Renpeng [2 ]
Hu, Jing [3 ]
Yin, Jian [1 ]
机构：
[1] Jiangnan Univ, Sch Biotechnol, Key Lab Carbohydrate Chem & Biotechnol, Minist Educ, Lihu Ave 1800, Wuxi 214122, Peoples R China
[2] Shandong Ctr Dis Control & Prevent, 16992 City Ten Rd, Jinan 250014, Shandong, Peoples R China
[3] Jiangnan Univ, Wuxi Sch Med, Lihu Ave 1800, Wuxi 214122, Peoples R China
[4] Ctr Dis Control & Prevent LanLing City, 1 City Huibao Rd, Lanling 276000, Shandong, Peoples R China
[5] Nankai Univ, TEDA Inst Biol Sci & Biotechnol, TEDA, 23 Hongda St, Tianjin 300457, Peoples R China
来源：
CARBOHYDRATE RESEARCH | 2022年 / 517卷
基金：
中国博士后科学基金; 国家重点研发计划; 中国国家自然科学基金;
关键词：
Escherichia coli; O-Antigen; Serotype; Structure; O-Antigen gene cluster; SOMATIC ANTIGENS; POLYSACCHARIDE; LIPOPOLYSACCHARIDE; PSEUDOMONADS;
D O I：
10.1016/j.carres.2022.108577
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The O-antigen is an important virulence factor involved in the survival, virulence and invasion of bacteria. The bacterial serological types are highly dependent on these surface-exposed and structurally unique O-antigen structures. In this work, the structure of O-antigen from an Escherichia coli strain 2017LL031 was elucidated as a hexasaccharide repeating unit: -> 3)-[beta-D-Glcp-(1 -> 2)]-alpha-L-Rhap-(1 -> 3)-[alpha-D-Quip-(1 -> 3)-alpha-D-GlcpA-(1 -> 2)]beta-L-Fucp-(1 -> 3)-beta-D-GlcpNAc-(1 ->, which is completely different from all known E. coli serogroups. The O-antigen gene cluster (O-AGC) of 2017LL031 was also analyzed and correlates well to its O-Ag. Moreover, the O-AGC of 2017LL031 was deleted and its role in O-Ag biosynthesis was confirmed experimentally. Taken together, our results present that a novel E.coli serotype 2017LL031 is identified.
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