Specificity clusters of MUC1-reactive mouse monoclonal antibodies

被引:13
作者
Hanisch, FG [1 ]
机构
[1] Univ Cologne, Fac Med, Inst Biochem 2, D-50931 Cologne, Germany
关键词
mucins; MUC1; monoclonal antibodies; carbohydrates; epitope analysis;
D O I
10.1159/000056511
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The ISOBM TD-4 Workshop antibodies 122-177 were grouped according to their reactivity with: (a) monomeric MUC1 peptide (TAP25); (b) the pentameric tandem repeat peptide (TR-5), both unglycosylated or as their Ga1NAc-substituted derivatives, and (c) the lactation or tumor-associated glycoforms of MUC1. The antibodies were grouped into nine clusters: Clusters 1-4 comprise those antibodies that bind to MUC1 tandem repeat peptide (monomeric) and define sequential epitopes differentially affected by glycosylation (Ga1NAc substitution) in the peptide motifs VTSA and GSTA. The ISOBM-163 antibody in cluster 5 defines Tn antigen as a site-specific glycotope within the tandem repeat peptide, The antibodies in clusters 6 and 7A recognize peptide epitopes within the tandem repeats, although these seem to be conformationally dependent on complex glycosylation or on oligomeric peptide repetition, Antibodies in clusters 7B bind to a conformational epitope not related to the tandem repeat peptide or to a carbohydrate epitope (cluster 7BC), Antibodies in cluster 8 showed a high selective binding to tumor-associated epitopes on MUC1 from mammary carcinoma cells, Cluster 9 antibodies were not reactive on any of the tested antigens and may be argued to lack MUC1 specificity, Alternatively, these antibodies could have a carbohydrate specificity not expressed by the MUC1 glycoforms tested in our studies.
引用
收藏
页码:111 / 117
页数:7
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